Localization of human type II procollagen gene (COL2A1) to chromosome 12.

DNA was prepared from 39 human-mouse somatic cell hybrid lines, and mouse and human parental cell lines. The DNA was digested to completion with EcoRI and Southern filters were prepared. These filters were hybridized at high stringency conditions to the human genomic subclone phHCol(II) A which corresponds to the human alpha 1(type II) procollagen gene (COL2A1). The mouse DNA yielded a single band at greater than 10 kb, whereas the human DNA had the expected single band at 4.8kb. Analyses of these human-mouse cell hybrids demonstrated that the human alpha 1(type II) procollagen gene segregates with chromosome 12.