Strom C M, Eddy R L, Shows T B
Somat Cell Mol Genet. 1984 Nov;10(6):651-5. doi: 10.1007/BF01535232.
DNA was prepared from 39 human-mouse somatic cell hybrid lines, and mouse and human parental cell lines. The DNA was digested to completion with EcoRI and Southern filters were prepared. These filters were hybridized at high stringency conditions to the human genomic subclone phHCol(II) A which corresponds to the human alpha 1(type II) procollagen gene (COL2A1). The mouse DNA yielded a single band at greater than 10 kb, whereas the human DNA had the expected single band at 4.8kb. Analyses of these human-mouse cell hybrids demonstrated that the human alpha 1(type II) procollagen gene segregates with chromosome 12.
从39个人-鼠体细胞杂交系以及小鼠和人亲本细胞系中提取DNA。用EcoRI将DNA完全消化，并制备Southern印迹滤膜。这些滤膜在高严谨条件下与人类基因组亚克隆phHCol(II)A杂交，该亚克隆对应于人α1（II型）前胶原基因（COL2A1）。小鼠DNA在大于10 kb处产生一条带，而人类DNA在4.8 kb处出现预期的单一条带。对这些人-鼠细胞杂种的分析表明，人α1（II型）前胶原基因与12号染色体分离。
