Adams Austin, Straseski Joely A, Lehman Christopher M, Pearson Lauren N
ARUP Laboratories, Salt Lake City, UT.
Pathology Department, University of Utah School of Medicine, Salt Lake City.
Lab Med. 2019 Apr 8;50(2):145-149. doi: 10.1093/labmed/lmy056.
Chemistry testing is requested for body fluid (BF) specimens despite the lack of assays approved by the US Food and Drug Administration (FDA). The criteria for categorizing fluids as transudate or exudate are not validated across analyzers.
To compare BF chemical analysis and classification by different analyzers.
We analyzed 10 pleural and 18 peritoneal fluids with corresponding plasma specimens using the Vitros 5,1 FS; Abbott ARCHITECT ci8200; and Roche Modular P platforms. Total protein (TP) and lactate dehydrogenase (LDH) were measured for pleural fluids. Light's criteria were applied. Albumin was measured for peritoneal specimens, and the plasma-ascites-albumin gradient was calculated.
TP results showed agreement. The Vitros LDH assay produced higher fluid:plasma ratios. Classification by Light's criteria resulted in 1 discrepancy (ARCHITECT). Albumin results showed agreement. There were 2 discrepant gradient interpretations (Vitros).
These data suggest that analyses of pleural and peritoneal fluids using these platforms are diagnostically interchangeable.
尽管缺乏美国食品药品监督管理局（FDA）批准的检测方法，但仍要求对体液（BF）标本进行化学检测。跨分析仪对液体进行漏出液或渗出液分类的标准尚未得到验证。
比较不同分析仪对BF的化学分析和分类。
我们使用Vitros 5,1 FS、雅培ARCHITECT ci8200和罗氏Modular P平台对10份胸水和18份腹水标本以及相应的血浆标本进行了分析。对胸水测定总蛋白（TP）和乳酸脱氢酶（LDH）。应用Light标准。对腹水标本测定白蛋白，并计算血浆-腹水白蛋白梯度。
TP结果显示具有一致性。Vitros的LDH检测产生的液/血比值更高。根据Light标准进行分类时出现了1例差异（ARCHITECT）。白蛋白结果显示具有一致性。有2例梯度解释存在差异（Vitros）。
这些数据表明，使用这些平台对胸水和腹水进行分析在诊断上具有可互换性。
