Department of Surgery, Helsingborg Hospital, Helsingborg, Sweden.
Department of Clinical Sciences, Lund University, Lund, Sweden.
Am J Clin Pathol. 2018 Oct 24;150(6):533-544. doi: 10.1093/ajcp/aqy083.
Immunohistochemical staining against thyroid transcription factor 1 (TTF-1) is often used to distinguish lung adenocarcinoma from squamous cell carcinoma and pulmonary metastasis.
TTF-1 expression was examined using the antibody clones 8G7G3/1, SPT24, and SP141 on tissue microarrays from 665 cases of resected lung cancers and 428 pulmonary metastases.
Most lung adenocarcinomas, 89%, 93%, and 93%, were positive with TTF-1 clones 8G7G3/1, SPT24, and SP141, respectively. The corresponding figures for lung squamous cell carcinomas were 0%, 6%, and 8%. In total, five (2%), 19 (7%), and 21 (8%) of the pulmonary metastases from colorectal adenocarcinomas were positive with clones 8G7G3/1, SPT24, and SP141, respectively. Other TTF-1-positive pulmonary metastases (n = 8) were thyroid, urothelial, pancreatic, small bowel, and cervix carcinomas.
TTF-1 expression in lung cancer and pulmonary metastases differs between clones, with 8G7G3/1 being more specific but less sensitive compared with SPT24 and SP141.
甲状腺转录因子 1（TTF-1）的免疫组织化学染色常用于区分肺腺癌与鳞状细胞癌和肺转移。
在 665 例肺癌和 428 例肺转移的组织微阵列上，使用克隆 8G7G3/1、SPT24 和 SP141 抗体检测 TTF-1 的表达。
大多数肺腺癌（分别为 89%、93%和 93%）对 TTF-1 克隆 8G7G3/1、SPT24 和 SP141 呈阳性，相应的肺鳞状细胞癌的比例为 0%、6%和 8%。在总共 5%（2%）、19%（7%）和 21%（8%）的结直肠腺癌肺转移中，克隆 8G7G3/1、SPT24 和 SP141 分别呈阳性。其他 TTF-1 阳性的肺转移（n=8）为甲状腺癌、尿路上皮癌、胰腺癌、小肠癌和宫颈癌。
肺癌和肺转移中 TTF-1 的表达在克隆之间存在差异，8G7G3/1 比 SPT24 和 SP141 更具特异性，但敏感性较低。
