Evans L H
Virology. 1986 Aug;153(1):122-36. doi: 10.1016/0042-6822(86)90013-9.
An immunological focus assay using monoclonal antibodies on live adherent in vitro cell lines was employed to detect and isolate different types of murine leukemia viruses (MuLVs) from spleen and thymus cells of young (less than 1 month of age) AKR/J mice. In agreement with earlier studies, ecotropic viruses were detected from cells of both tissues in all mice tested, although only trace levels of ecotropic MuLV infectious centers were found with thymus cells from mice of this age. Polytropic MuLVs were not detected in mice less than 3 weeks of age; however, between the ages of 3 and 4 weeks, polytropic viruses were detectable in assays of spleen cells from 50% of the mice. No polytropic MuLVs were detected in assays of thymocytes from any mice of this age. Several polytropic MuLVs obtained from spleens of young mice were further characterized. All of the isolates were infectious for both mink and SC-1 (feral mouse) cells, and exhibited interference properties typical of polytropic MuLVs. However, none of the viruses induced obvious cytopathic effects (CPE) on mink cells. All of the viruses appeared antigenically similar with regard to their reactivities to a panel of 12 monoclonal antibodies directed at envelope antigens of polytropic MuLVs. RNase T1-resistant oligonucleotide analysis of a polytropic MuLV from a 26-day-old mouse indicated that its entire env gene was derived from nonecotropic sequences while the remainder of its genome was indistinguishable from the ecotropic parent. The isolate thus exhibited a genome structure typical of Class II polytropic MuLVs and is the first example of this type of MuLV isolated from AKR/J mice. Examination of polytropic MuLVs derived from the spleens and thymuses of 5- to 6-month-old mice indicated that only 2 of 10 isolates examined induced CPE on mink cells. Furthermore, most of the CPE-negative viruses isolated from spleen and thymus cells of these mice exhibited in vitro host ranges and antigenic reactivities similar to isolates from young mice, suggesting that this type of polytropic MuLV may originate in the spleen, subsequently spread to other tissues, and persist throughout the preleukemic period. The detection of polytropic viruses in a large proportion of very young mice is in contrast to previous studies which have not detected polytropic virus production in AKR mice less than 5 to 6 months of age.(ABSTRACT TRUNCATED AT 250 WORDS)
采用针对体外贴壁活细胞系的单克隆抗体免疫聚焦试验，从幼龄（小于1月龄）AKR/J小鼠的脾脏和胸腺细胞中检测并分离不同类型的鼠白血病病毒（MuLVs）。与早期研究一致，在所有检测的小鼠中，两种组织的细胞均检测到嗜亲性病毒，不过在这个年龄段小鼠的胸腺细胞中仅发现微量的嗜亲性MuLV感染中心。小于3周龄的小鼠未检测到多嗜性MuLVs；然而，在3至4周龄之间，50%的小鼠脾脏细胞检测中可检测到多嗜性病毒。这个年龄段的任何小鼠胸腺细胞检测中均未检测到多嗜性MuLVs。对从幼龄小鼠脾脏中获得的几种多嗜性MuLVs进行了进一步鉴定。所有分离株对水貂和SC-1（野生小鼠）细胞均具有感染性，并表现出多嗜性MuLVs典型的干扰特性。然而，这些病毒均未对水貂细胞诱导明显的细胞病变效应（CPE）。就针对多嗜性MuLV包膜抗原的一组12种单克隆抗体的反应性而言，所有病毒在抗原性上似乎相似。对一只26日龄小鼠的多嗜性MuLV进行核糖核酸酶T1抗性寡核苷酸分析表明，其整个env基因源自非嗜亲性序列，而其基因组的其余部分与嗜亲性亲本无法区分。该分离株因此表现出典型的II类多嗜性MuLV基因组结构，是从AKR/J小鼠中分离出的此类MuLV的首个实例。对5至6月龄小鼠脾脏和胸腺来源的多嗜性MuLVs进行检测表明，所检测的10个分离株中只有2个对水貂细胞诱导CPE。此外，从这些小鼠的脾脏和胸腺细胞中分离出的大多数CPE阴性病毒在体外的宿主范围和抗原反应性与幼龄小鼠的分离株相似，这表明这种类型的多嗜性MuLV可能起源于脾脏，随后扩散到其他组织，并在白血病前期持续存在。在很大比例的幼龄小鼠中检测到多嗜性病毒，这与之前未在小于5至6月龄的AKR小鼠中检测到多嗜性病毒产生的研究形成对比。（摘要截断于250字）
