College of Life Science and Engineering, Foshan University, Guangdong, China; Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, Canada.
Department of Animal and Poultry Science, College of Agriculture and Bioresources, University of Saskatchewan, Canada.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jan 5;206:413-420. doi: 10.1016/j.saa.2018.08.022. Epub 2018 Aug 20.
Recently, advanced synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-IMS) has been developed as a rapid, direct, non-destructive and bioanalytical technique. To date, there has been very little application of this technique to study the molecular structure make-up in pulse seeds. Thus, the objectives of this study were to detect the interactive association between protein molecular structure and nutrient availability of newly developed Vicia faba varieties. Two different varieties of faba beans (CDC Snowdrop = low-tannin variety; vs. FB9-4 = high-tannin variety) were selected for this study. The molecular spectra data were collected by using SR-IMS. The ratio of both amide I to II area and height were higher (P < 0.01), while the ratio of α-helix to β-sheet was lower (P < 0.05) in CDC Snowdrop compared to FB9-4. The crude protein (CP) content and the predicted truly digestible nutrients as well as the bioenergy values did not vary between two varieties. The CDC Snowdrop had exhibited a higher (P < 0.01) rapidly degradable CP fraction (75.99 vs. 71.45% on CP) and a lower (P < 0.01) moderately degradable CP fraction (19.43 vs. 22.85% on CP), resulting in a higher (P < 0.01) rumen degradable protein and a lower (P < 0.01) rumen undegradable protein content than that of FB9-4 variety. However, the total supply of digestible rumen undegraded feed protein was higher (P < 0.05) in FB9-4 than CDC Snowdrop. Strong positive correlations were found between the ratio of α-helix to β-sheet and CP contents (R = 0.86, P < 0.01) as well as the truly digestible CP contents (R = 0.83, P < 0.01); respectively. In conclusion, the results of this study reveal that the protein are metabolized differently between different type of faba bean varieties and the advanced SR-IMS molecular spectroscopy can be used to rapidly delineate protein molecular structure motifs along with their nutritive value in ruminant livestock system.
最近，基于同步辐射的傅里叶变换红外微光谱（SR-IMS）已被开发为一种快速、直接、无损和生物分析技术。迄今为止，这项技术在研究脉冲种子的分子结构组成方面的应用非常有限。因此，本研究的目的是检测新开发的蚕豆品种中蛋白质分子结构与营养可用性之间的相互关联。本研究选择了两种不同的蚕豆品种（CDC Snowdrop=低单宁品种；与 FB9-4=高单宁品种）。使用 SR-IMS 采集分子光谱数据。与 FB9-4 相比，CDC Snowdrop 的酰胺 I 与 II 面积比和高度比更高（P<0.01），而α-螺旋与β-折叠比更低（P<0.05）。粗蛋白（CP）含量和预测的真正可消化养分以及生物能值在两个品种之间没有差异。CDC Snowdrop 表现出更高的（P<0.01）快速可降解 CP 分数（CP 上的 75.99%与 71.45%）和更低的（P<0.01）中速可降解 CP 分数（CP 上的 19.43%与 22.85%），导致瘤胃可降解蛋白更高（P<0.01）和瘤胃未降解蛋白含量更低（P<0.01）比 FB9-4 品种。然而，FB9-4 的可消化瘤胃未降解饲料蛋白总供应量高于 CDC Snowdrop（P<0.05）。α-螺旋与β-折叠比与 CP 含量（R=0.86，P<0.01）以及真正可消化 CP 含量（R=0.83，P<0.01）之间存在很强的正相关关系。总之，本研究结果表明，不同类型的蚕豆品种之间蛋白质的代谢方式不同，先进的 SR-IMS 分子光谱技术可用于快速描绘反刍家畜系统中蛋白质分子结构特征及其营养价值。
