Zhang Yingyu, Xu Hongqin, Chi Xiumei, Fan Yuxiang, Shi Ying, Niu Junqi
Department of Hepatology, The First Hospital of Jilin University, Changchun, Jilin, China.
Medicine (Baltimore). 2018 Aug;97(35):e11781. doi: 10.1097/MD.0000000000011781.
Human Cripto-1 (CR-1), a member of the epidermal growth factor-Cripto-1/FRL-1/Cryptic protein family (EGF-CFC), is highly expressed in a variety of human cancers. We aimed to detect serum CR-1 level in liver diseases especially in hepatocellular carcinoma (HCC) patients.
Serum CR-1 level was Sandwich-type enzyme-linked immuno sorbent assay (ELISA) detected in 330 patients with liver diseases including HCC, cirrhosis, and chronic hepatitis and 50 volunteers without hepatitis B virus (HBV) or hepatitis C virus (HCV) infection as control.
The serum CR-1 level was significantly higher in HCC patients than volunteer controls and it was also significantly higher in HBV-related HCC than HCV-related HCC. In addition, serum CR-1 level was correlated with serum alpha-feto-protein (AFP) in HBV-related HCC patients. The serum CR-1 was also higher in cirrhosis and chronic hepatitis than volunteer controls. The serum CR-1 in HBV-related cirrhosis was higher than chronic hepatitis B, but there was no significant difference between HCV-related cirrhosis and chronic hepatitis C.
Serum CR-1 was higher in HCC patients and might serve as a complementary biomarker to clinical diagnosis of HBV-related HCC. The high level of serum CR-1 in HBV-related liver disease might be partly attributed to HBV infection.
人CRIPTO-1（CR-1）是表皮生长因子-Cripto-1/FRL-1/隐窝蛋白家族（EGF-CFC）的成员，在多种人类癌症中高表达。我们旨在检测肝病尤其是肝细胞癌（HCC）患者的血清CR-1水平。
采用夹心型酶联免疫吸附测定（ELISA）检测330例肝病患者（包括HCC、肝硬化和慢性肝炎患者）以及50例未感染乙肝病毒（HBV）或丙肝病毒（HCV）的志愿者（作为对照）的血清CR-1水平。
HCC患者的血清CR-1水平显著高于志愿者对照，且HBV相关HCC患者的血清CR-1水平显著高于HCV相关HCC患者。此外，HBV相关HCC患者的血清CR-1水平与血清甲胎蛋白（AFP）相关。肝硬化和慢性肝炎患者的血清CR-1水平也高于志愿者对照。HBV相关肝硬化患者的血清CR-1水平高于慢性乙型肝炎患者，但HCV相关肝硬化和慢性丙型肝炎患者之间无显著差异。
HCC患者的血清CR-1水平较高，可能作为HBV相关HCC临床诊断的补充生物标志物。HBV相关肝病患者血清CR-1水平升高可能部分归因于HBV感染。
