Mao Yantao, Tian Mei, Pan Bo, Zhu Qingshan, Li Paiyun, Liu Hongmei, Liu Weipeng, Dai Ningtao, Yu Lili, Tian Yuan
Department of Oncology, Yantaishan Hospital of Shandong Province, Zhifu District, Yantai City Respiratory Department, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong Department of Lung Transplantation, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou Department of Radiotherapy Oncology, Anyang Cancer Hospital of Henan Province, Anyang, Henan Division of Etiology, Peking University Cancer Hospital and Institute, Beijing Department of Radiation Oncology, Shandong Provincial Qianfoshan Hospital Affiliated to Shandong University, Jinan, Shandong, China.
Medicine (Baltimore). 2018 Aug;97(35):e12021. doi: 10.1097/MD.0000000000012021.
To explore the relationship between mouse double minute 2 binding protein (MTBP) and the prognosis of cancer patients, a databank-based reanalysis was conducted and a clinical observation about lung adenocarcinoma was taken to verify the result of data analysis.We reanalyzed all the downloaded data in order to make a conclusion about the relationship between MTBP and the prognosis of cancer patients. At last, we collected 112 lung cancer patients with MTBP information to verify the results of data analysis (GSE30219).The overall Kaplan-Meier curve results of 6 eligible data groups were shown in Fig. 1. The Kaplan-Meier curve result of GSE16011 was shown in Fig. 1A (concordance index = 59.48, Log-Rank Equal Curves [P = 5.942e-05], R = 0.045/1, risk groups hazard ratio = 1.69 [conf. int. 1.3-2.9], P = 7.344e-05), while the stratification results were displayed independently in Figs. 2 and 3. The similar results could be seen in other 5 data groups. The tissue sections of 112 patients with lung adenocarcinoma were collected and immunohistochemically stained. The hyper expression rate of MTBP in adenocarcinoma was 23.21% (26/112). The results showed that patients with hyper expression of MTBP had significantly worse prognosis than the control group, and the survival curves were clearly separated from each other (Fig. 4B, P = .000).Hyper expression of MTBP maybe an adverse event for the survival of some cancer patients, especially in glioblastoma, kidney cancer, and lung cancer patients, which has been verified in 112 lung cancer patients with MTBP status.
为了探究小鼠双微体2结合蛋白（MTBP）与癌症患者预后之间的关系，我们进行了基于数据库的再分析，并对肺腺癌进行了临床观察以验证数据分析结果。我们重新分析了所有下载的数据，以便得出MTBP与癌症患者预后之间关系的结论。最后，我们收集了112例有MTBP信息的肺癌患者以验证数据分析结果（GSE30219）。6个符合条件的数据组的总体Kaplan-Meier曲线结果如图1所示。GSE16011的Kaplan-Meier曲线结果如图1A所示（一致性指数=59.48，对数秩相等曲线[P=5.942e-05]，R=0.045/1，风险组风险比=1.69[置信区间1.3-2.9]，P=7.344e-05），而分层结果分别显示在图2和图3中。在其他5个数据组中也可以看到类似结果。收集了112例肺腺癌患者的组织切片并进行免疫组织化学染色。MTBP在腺癌中的高表达率为23.21%（26/112）。结果显示，MTBP高表达的患者预后明显比对照组差，生存曲线明显分离（图4B，P=0.000）。MTBP的高表达可能对一些癌症患者的生存是一个不良事件，尤其是在胶质母细胞瘤、肾癌和肺癌患者中，这在112例有MTBP状态的肺癌患者中得到了验证。
