Kobayashi M, Nakatsukasa H, Watanabe A, Yamauchi Y, Fujiwara M, Hashimoto M, Watanabe M, Higashi T, Nagashima H
Acta Med Okayama. 1986 Jun;40(3):179-82. doi: 10.18926/AMO/31933.
Type V collagen-degrading enzyme activity was detected as a metalloprotease acting at neutral pH in the human liver. Type V collagen extracted from human placenta and labeled with [1-14C] acetic anhydride was used as the substrate in the assay. Four major degradation products with relatively high molecular weights were observed upon polyacrylamide gel electrophoresis of the incubation mixture of type V collagen and liver homogenate. The significance of the measurement of this enzyme activity was discussed in relation to the clarification of the mechanism of liver fibrosis.
在人肝脏中检测到V型胶原降解酶活性，其作为一种在中性pH下起作用的金属蛋白酶。从人胎盘中提取并用[1-¹⁴C]乙酸酐标记的V型胶原用作测定中的底物。对V型胶原与肝脏匀浆的孵育混合物进行聚丙烯酰胺凝胶电泳后，观察到四种相对高分子量的主要降解产物。结合肝纤维化机制的阐明，讨论了该酶活性测定的意义。
