Hashimoto N, Kobayashi M, Watanabe A, Higashi T, Tsuji T
First Department of Internal Medicine, Okayama University Medical School, Japan.
Acta Med Okayama. 1988 Feb;42(1):49-52. doi: 10.18926/AMO/31036.
Type IV collagen-degrading enzyme activity was detected in human serum. Serum was preincubated with 4-aminophenylmercuric acetate and trypsin to activate the enzyme prior to assay. Type IV collagen, purified from human placentas and radiolabeled with [1-14C] acetic anhydride, was used as the substrate. The enzyme activity was measured at pH 7.5 and inhibited by treatment with ethylenediaminetetraacetic acid or heat. The assay of type IV collagen-degrading enzyme in human serum might be useful for estimating the degradation of type IV collagen.
在人血清中检测到IV型胶原降解酶活性。在测定前，血清先与乙酸4-氨基苯汞和胰蛋白酶预孵育以激活该酶。从人胎盘中纯化并用[1-¹⁴C]乙酸酐进行放射性标记的IV型胶原用作底物。在pH 7.5条件下测量酶活性，并用乙二胺四乙酸处理或加热可抑制该活性。检测人血清中的IV型胶原降解酶可能有助于评估IV型胶原的降解情况。
