Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Khawaja Fareed Campus, Railway Road, Bahawalpur, Punjab, 63100, Pakistan.
Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, Pakistan.
AAPS PharmSciTech. 2018 Oct;19(7):3199-3209. doi: 10.1208/s12249-018-1131-z. Epub 2018 Aug 31.
The purpose of the study was to synthesize and characterize a new form of topical membranes as chitosan-based hydrogel membranes for bacterial skin infections. The polymeric membranes were synthesized by modification in free radical solution polymerization technique. High molecular weight (HMW) chitosan polymer was cross-linked with monomer 2-acrylamido-2-methylpropane sulfonic acid (AMPS) through cross-linker N,N-methylenebisacrylamide (MBA). Mupirocin, an antibiotic, was used as model drug. The polymeric membranes were prepared in spherical form that found stable and elastic. Characterization of hydrogel membranes was performed by FTIR, SEM, DSC, TGA, swelling behavior, drug release, irritation study, and ex vivo drug permeation and deposition study. Structural and thermal studies confirmed the formation of new polymeric network with enhanced stability of hydrogel membranes. Permeation flux of drug from optimized formulation through rabbit's skin assessed by using Franz cell was up to 104.09 μg cm h. Furthermore, hydrogel membrane has significant retention of drug in skin up to 2185 μg 1.5 cm. Draize patch test confirmed the synthesized hydrogels as non-irritant to skin. The preparation of a topical membrane with improved antibacterial activity within controlled release manner is desirable for the advancement and treatment of skin diseases.
本研究的目的是合成并表征一种新的局部膜形式，即壳聚糖基水凝胶膜，用于治疗细菌性皮肤感染。聚合膜是通过自由基溶液聚合技术进行修饰合成的。高分子量（HMW）壳聚糖聚合物通过交联剂 N,N-亚甲基双丙烯酰胺（MBA）与单体 2-丙烯酰胺基-2-甲基丙磺酸（AMPS）交联。将抗生素莫匹罗星用作模型药物。聚合膜以球形形式制备，发现其具有稳定性和弹性。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、差示扫描量热法（DSC）、热重分析（TGA）、溶胀行为、药物释放、刺激性研究以及体外药物渗透和沉积研究对水凝胶膜进行了表征。结构和热研究证实了新的聚合网络的形成，从而增强了水凝胶膜的稳定性。通过Franz 细胞评估，从优化配方中通过兔皮的药物渗透通量高达 104.09μgcmh。此外，水凝胶膜在皮肤中的药物滞留量高达 2185μg1.5cm。Draize 贴片试验证实合成水凝胶对皮肤无刺激性。制备具有改善的抗菌活性和控制释放方式的局部膜对于皮肤疾病的治疗和改善是理想的。
