金华猪和长白猪肌肉内脂肪组织中 microRNAs 的比较。

Comparison of microRNAs in the intramuscular adipose tissue from Jinhua and Landrace pigs.

机构信息

College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, Henan, China.

Department of Pathobiology, Faculty of Veterinary and Animal Sciences, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan.

出版信息

J Cell Biochem. 2019 Jan;120(1):192-200. doi: 10.1002/jcb.27298. Epub 2018 Sep 1.

DOI:10.1002/jcb.27298

PMID:30171716

Abstract

MicroRNA (miRNA) is critically involved in lipogenesis occurring in various body parts of humans and animals. In this study, to further investigate the role and distribution of miRNA in porcine intramuscular adipose tissue, small RNAs were extracted from Jinhua and Landrace pigs to identify the expression profiles of miRNAs. miRNA expression profiles revealed that 558 miRNAs including 287 known and 271 novel miRNAs were identified, and 220 of them showed differential expression in the pigs. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis suggested that the target genes of the differentially expressed miRNAs were involved in fatty metabolism. In conclusion, the current study reveals the active participation of miRNAs in the regulation of adipogenesis in the intramuscular adipose tissue of Jinhua and Landrace pigs.

摘要

微小 RNA（miRNA）在人类和动物的各种身体部位发生的脂肪生成中起着至关重要的作用。在这项研究中，为了进一步研究 miRNA 在猪肌肉内脂肪组织中的作用和分布，从小金华猪和长白猪中提取了小 RNA，以鉴定 miRNA 的表达谱。miRNA 表达谱显示，鉴定出了 558 种 miRNA，包括 287 种已知 miRNA 和 271 种新 miRNA，其中 220 种在猪中表现出差异表达。基因本体论和京都基因与基因组百科全书途径富集分析表明，差异表达 miRNA 的靶基因参与脂肪代谢。总之，本研究揭示了 miRNA 在金华猪和长白猪肌肉内脂肪组织中脂肪生成的调节中的积极参与。