College of Biological and Chemical Engineering, Jiaxing University, Jiaxing Zhejiang, 314000, China.
College of Agronomy and Biotechnology, Hebei Normal University of Science and Technology, Qin Huangdao Hebei, 066000, China.
BMC Genomics. 2023 Jun 27;24(1):360. doi: 10.1186/s12864-023-09477-0.
As components of white adipose tissue, porcine intramuscular (IM) and subcutaneous (SC) adipocytes undergo similar differentiation and adipogenesis processes. However, the adipogenic capacity of IM adipocytes is weaker than that of SC adipocytes. Identifying key regulators underlying this difference between IM and SC adipocytes will benefit pig breeding.
In this study, we used BGISEQ-500 sequencing technology to analyze the expression of small RNAs in primary cultured IM and SC adipocytes on day 8 after adipogenic induction, and found 32-fold higher miR-196b-3p expression, as well as 8-fold lower miR-450b-3p expression in IM adipocytes than in SC adipocytes. Functional studies revealed that miR-196b-3p inhibits adipogenesis by targeting CD47 via the AMPK signaling pathway, and its effect was attenuated by the specific p-AMPKα activator AICAR. We also found that miR-450b-3p promotes adipogenesis by targeting SIRT1 via the Wnt/β-catenin signaling pathway, and its effect was weakened by the Wnt/β-catenin signaling activator LiCl.
Our findings suggest that miR-196b-3p and miR-450b-3p are novel key regulatory factors that play opposite roles in porcine adipogenesis, helping us decipher the regulatory differences between porcine IM and SC fat deposition.
作为白色脂肪组织的组成部分，猪的肌内（IM）和皮下（SC）脂肪细胞经历相似的分化和脂肪生成过程。然而，IM 脂肪细胞的脂肪生成能力比 SC 脂肪细胞弱。鉴定 IM 和 SC 脂肪细胞之间这种差异的关键调节因子将有益于猪的繁殖。
在这项研究中，我们使用 BGISEQ-500 测序技术分析了诱导脂肪生成 8 天后原代培养的 IM 和 SC 脂肪细胞中小 RNA 的表达，发现 IM 脂肪细胞中 miR-196b-3p 的表达水平高出 32 倍，miR-450b-3p 的表达水平低 8 倍。功能研究表明，miR-196b-3p 通过 AMPK 信号通路靶向 CD47 抑制脂肪生成，其作用可被 AMPKα 的特异性激活剂 AICAR 减弱。我们还发现，miR-450b-3p 通过 Wnt/β-catenin 信号通路靶向 SIRT1 促进脂肪生成，其作用可被 Wnt/β-catenin 信号激活剂 LiCl 减弱。
我们的研究结果表明，miR-196b-3p 和 miR-450b-3p 是猪脂肪生成中发挥相反作用的新的关键调节因子，有助于我们揭示猪 IM 和 SC 脂肪沉积的调节差异。
