Textile Pollution Controlling Engineering Center of Ministry of Environmental Protection, College of Environmental Science and Engineering, Donghua University, Shanghai 201620, PR China.
Textile Pollution Controlling Engineering Center of Ministry of Environmental Protection, College of Environmental Science and Engineering, Donghua University, Shanghai 201620, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China.
Bioresour Technol. 2018 Dec;269:153-161. doi: 10.1016/j.biortech.2018.08.100. Epub 2018 Aug 25.
In this study, an expanded granular sludge blanket (EGSB) reactor was used for the treatment of low-strength domestic sewage and the sludge granulation process was systematically investigated. At an optimized hydraulic retention time (HRT) of 5 h, up-flow velocity (V) of 1.9 m/h, and organic loading rate (OLR) of 2.16 kg COD/m/d, the average COD removal efficiency was 71.5 ± 2.3%. Completely granular sludge can be observed after 107 d of continuous operation. Analysis of the distribution and composition of the extracellular polymeric substances (EPS) indicates that the tightly bound EPS (TB-EPS) content shows an increasing trend, while the loosely bound EPS (LB-EPS) content did not significantly alter after the granular sludge was formed. The three-dimensional excitation-emission matrix technique (3D-EEM) confirms that aromatic protein-like substances are of key importance to sludge granulation. High-throughput sequencing analysis indicates that the metabolism shifted from hydrogenotrophic (Methanobaterium) to aceticlastic methanogens (Methanosaeta) during sludge granulation.
在这项研究中，采用膨胀颗粒污泥床（EGSB）反应器处理低浓度生活污水，并系统地研究了污泥颗粒化过程。在优化的水力停留时间（HRT）为 5 小时、上流速度（V）为 1.9 米/小时和有机负荷率（OLR）为 2.16 千克 COD/m/d 的条件下，平均 COD 去除效率为 71.5±2.3%。连续运行 107 天后，可以观察到完全颗粒状的污泥。对胞外聚合物（EPS）分布和组成的分析表明，紧密结合的 EPS（TB-EPS）含量呈上升趋势，而形成颗粒污泥后松散结合的 EPS（LB-EPS）含量没有明显变化。三维激发-发射矩阵技术（3D-EEM）证实芳香族蛋白样物质对污泥颗粒化至关重要。高通量测序分析表明，在污泥颗粒化过程中，代谢从氢营养型（Methanobaterium）转变为乙酸营养型甲烷菌（Methanosaeta）。
