Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China.
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Talanta. 2018 Dec 1;190:263-268. doi: 10.1016/j.talanta.2018.07.071. Epub 2018 Jul 25.
We describe a novel biosensing strategy for sensitive detection of methylamphetamine (MAMP) based on surface enhanced Raman scattering (SERS) by the mediation of spacing between 4-mercaptobenzoic acid (4-MBA) labeled Au@Ag core-shell nanoparticles (Au@Ag). To achieve a favorable SERS substrate, Au@Ag shell-core nanoparticle was synthesized with seeds growth method and well characterized by SEM, TEM and UV-vis spectrometer. The uniform Au@Ag shows an excellent dispersion ability for SERS detection. Under the optimized conditions, the novel biosensor shows a good logarithm linear correlation with the concentration of MAMP ranging from 0.5 ppb to 40 ppb (R = 0.986), with a limit of detection at 0.16 ppb of MAMP (3σ). Furthermore, our biosensors hold an excellent selectivity, demonstrated by the negligible interference from the detection of other illicit drugs and metabolites. The concentrations determined with our biosensor from spiked MAMP in human urine sample fell within the same range with the results from mass spectrometry. This indicates that our sensor has a clear potential for the rapid detection of illicit drug in real samples.
我们描述了一种基于表面增强拉曼散射（SERS）的新型生物传感策略，用于通过 4-巯基苯甲酸（4-MBA）标记的 Au@Ag 核壳纳米粒子（Au@Ag）之间的间距介导来灵敏检测甲基苯丙胺（MAMP）。为了获得有利的 SERS 基底，采用种子生长法合成了 Au@Ag 壳核纳米粒子，并通过 SEM、TEM 和紫外可见分光光度计进行了很好的表征。均匀的 Au@Ag 表现出优异的 SERS 检测分散能力。在优化条件下，新型生物传感器对 MAMP 的浓度在 0.5 ppb 至 40 ppb（R = 0.986）范围内呈现良好的对数线性相关性，MAMP 的检测限为 0.16 ppb（3σ）。此外，我们的生物传感器具有出色的选择性，通过检测其他非法药物和代谢物的干扰可以明显看出。从人尿样中加入 MAMP 后，我们的生物传感器测定的浓度与质谱法的结果在相同范围内。这表明我们的传感器在实际样品中对非法药物的快速检测具有明显的潜力。
