School of Electronic Engineering, Guangxi University of Science and Technology, No.2, Wenchang Road, Liuzhou City, 545006, Guangxi, China.
Faculty of Computing, Engineering & Science, University of South Wales, Wales, CF37 1DL, UK.
Mikrochim Acta. 2024 Oct 12;191(11):664. doi: 10.1007/s00604-024-06756-9.
A method for detecting methamphetamine (MET), ketamine (KET), and morphine (MOP) molecules is presented using a reusable substrate based on SERS. The SERS substrate was prepared by etching the Au/Ag alloy film to synthesize a nanoporous Au membrane (AuNPM). By optimizing the preparation conditions and using rhodamine 6G (R6G) as an analyte, the AuNPM exhibited good SERS performance with a limit of detection (LOD) of 10 mol L. A competitive immunoassay category has been applied to the detection of MET, KET, and MOP. The MET, KET, and MOP antigens were functionalized on the surface of the AuNPM to specifically bind to the related drug antibodies. The Au nanoparticles (AuNPs) modified with 4-mercaptobenzoic acid (4-MBA) and antibodies against MET, KET, and MOP were used as nanotags. The 4-MBA served as the reporting molecule and drug antibodies were used to bind to free drug molecules in the target solution. The mixture of nanotags and target solution was dropped onto the antigen-modified AuNPM (antigen/AuNPM), and the free nanotags bind to the antigen/AuNPM. By comparing the SERS intensity of 4-MBA with the presence or absence of drug molecules, the drugs were qualitatively and quantitatively identified. Through this category, the LODs for detecting MET, KET, and MOP were 0.1, 1, and 1 ng mL, respectively. This study proposes an effective method for constructing SERS-based detection of drug molecules with good potential for practical applications.
本文提出了一种基于表面增强拉曼散射（SERS）的可重复使用基底检测甲基苯丙胺（MET）、氯胺酮（KET）和吗啡（MOP）分子的方法。该 SERS 基底是通过刻蚀 Au/Ag 合金膜合成纳米多孔金膜（AuNPM）制备的。通过优化制备条件并使用罗丹明 6G（R6G）作为分析物，AuNPM 表现出良好的 SERS 性能，检测限（LOD）为 10 mol L。竞争性免疫测定类别已应用于 MET、KET 和 MOP 的检测。将 MET、KET 和 MOP 抗原功能化到 AuNPM 表面，以特异性结合相关药物抗体。用 4-巯基苯甲酸（4-MBA）和针对 MET、KET 和 MOP 的抗体修饰的金纳米颗粒（AuNPs）用作纳米标签。4-MBA 用作报告分子，药物抗体用于结合目标溶液中游离的药物分子。将纳米标签和目标溶液的混合物滴加到抗原修饰的 AuNPM（抗原/AuNPM）上，游离的纳米标签与抗原/AuNPM 结合。通过比较存在或不存在药物分子时 4-MBA 的 SERS 强度，对药物进行定性和定量鉴定。通过这种方法，检测 MET、KET 和 MOP 的 LOD 分别为 0.1、1 和 1 ng mL。本研究提出了一种基于 SERS 的有效检测药物分子的方法，具有良好的实际应用潜力。
