Sotnikov O S, Aliev A A
Arkh Anat Gistol Embriol. 1986 May;90(5):21-7.
Structural-functional reconstructions of the frog autonomic interneuronal synapsis have been studied at its activation with endogenic acetylcholine under conditions of acetylcholinesterase suppression. The investigation has been performed with preparations of isolated sympathetic trunk of Rana temporaria treated with armine (5 X 10(-6) M) and subjected to electrostimulation (5 imp/sec) up to a complete block of the synaptic transmission. Certain structural changes are revealed in the axo-somatic synapses, demonstrating an increased adhesive properties of the membranes, ("hatch-like" membranes, numerous submembranous aggregates, aggregates of the intercellular cleft and neuronal-glial contacts). In the terminals changed according to the "light type", with poorly manifested changes, light synaptic vesicles loose their spheric form, their diameter decreases. In the boutons with more intensive changes, the vesicles gradually change into the mass of cluster-floccular material. In the boutons with intensively manifested disorders in the ultrastructure, a complete destruction of the light vesicles is observed. The great part of the ganglionic neuron bodies changes according the "dark type". In their neuroplasm a great amount of subsuperficial cisterns of the endoplasmic reticulum and formation of powerful fasciculi of microfilaments are noted to appear.
在乙酰胆碱酯酶受抑制的条件下，用内源性乙酰胆碱激活青蛙自主神经元突触时，对其结构 - 功能重建进行了研究。该研究使用了经阿米宁（5×10⁻⁶ M）处理的林蛙分离交感干标本，并进行电刺激（5次脉冲/秒）直至突触传递完全阻断。轴体突触中出现了某些结构变化，表现为膜的黏附特性增强（“舱口样”膜、大量膜下聚集体、细胞间裂隙聚集体和神经胶质接触）。在根据“轻型”改变的终末中，变化表现不明显，轻型突触小泡失去球形形态，直径减小。在变化更明显的终扣中，小泡逐渐变成团簇状絮状物质。在超微结构紊乱明显的终扣中，观察到轻型小泡完全破坏。大部分神经节神经元胞体根据“重型”发生变化。在其神经质中，内质网的大量浅表池出现，微丝形成强大的束状结构。
