Hortin G, Green E D, Baenziger J U, Strauss A W
Biochem J. 1986 Apr 15;235(2):407-14. doi: 10.1042/bj2350407.
Several human glycoproteins, including alpha 1-antitrypsin, alpha 1-acid glycoprotein, transferrin, caeruloplasmin and alpha 2HS-glycoprotein, synthesized by the hepatoma-derived cell line HepG2 were observed to contain covalently linked sulphate. These proteins were estimated to contain about 0.1 mol of sulphate/mol of protein. The most abundant of the sulphated glycoproteins, alpha 2HS-glycoprotein, was analysed in detail. All of the sulphate on this protein was attached to N-linked oligosaccharides which contained sialic acid and resisted release by endoglycosidase H. Several independent analytical approaches established that approx. 10% of the molecules of alpha 2HS-glycoprotein contained sulphate. Our results suggest that a number of human plasma proteins contain small amounts of sulphate linked to oligosaccharides.
通过肝癌衍生细胞系HepG2合成的几种人类糖蛋白，包括α1 -抗胰蛋白酶、α1 -酸性糖蛋白、转铁蛋白、铜蓝蛋白和α2HS -糖蛋白，被观察到含有共价连接的硫酸盐。据估计，这些蛋白质每摩尔蛋白质含有约0.1摩尔硫酸盐。对硫酸盐化糖蛋白中含量最丰富的α2HS -糖蛋白进行了详细分析。该蛋白质上所有的硫酸盐都连接到含有唾液酸且对内切糖苷酶H有抗性的N -连接寡糖上。几种独立的分析方法证实，约10%的α2HS -糖蛋白分子含有硫酸盐。我们的结果表明，许多人类血浆蛋白含有少量与寡糖连接的硫酸盐。
