Hortin G, Natowicz M, Pierce J, Baenziger J, Parsons T, Boime I
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7468-72. doi: 10.1073/pnas.78.12.7468.
Chemical analyses have previously detected sulfate linked to the oligosaccharides of lutropin isolated from bovine and human pituitaries. To determine whether lutropin could be metabolically labeled with sulfate, isolated bovine and rat pituitaries were incubated with [35S]sulfate. In both species, two major labeled products were immunoprecipitated with antisera specific to lutropin subunits. Incorporation into the subunits occurred posttranslationally since it was not blocked by cycloheximide, which did, however, block the incorporation of radiolabeled methionine. Metabolic labeling with [35S]sulfate provides a valuable approach for examining the biosynthetic processing of lutropin and the physiological role of sulfate in this hormone.
化学分析先前已检测到与从牛和人垂体中分离出的促黄体生成素寡糖相连的硫酸盐。为了确定促黄体生成素是否可以被硫酸盐进行代谢标记，将分离出的牛和大鼠垂体与[35S]硫酸盐一起孵育。在这两个物种中，用促黄体生成素亚基特异性抗血清免疫沉淀出两种主要的标记产物。亚基中的掺入发生在翻译后，因为它不受环己酰亚胺的阻断，然而，环己酰亚胺确实阻断了放射性标记甲硫氨酸的掺入。用[35S]硫酸盐进行代谢标记为研究促黄体生成素的生物合成过程以及硫酸盐在这种激素中的生理作用提供了一种有价值的方法。
