GET 通路介导的尾部锚定蛋白生物发生的结构视角
A structural perspective on tail-anchored protein biogenesis by the GET pathway.
机构信息
Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
Department of Biochemistry and Molecular Biology, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
出版信息
Curr Opin Struct Biol. 2018 Aug;51:195-202. doi: 10.1016/j.sbi.2018.07.009. Epub 2018 Aug 30.
Abstract
Many tail-anchored (TA) membrane proteins are targeted to and inserted into the endoplasmic reticulum (ER) by the `guided entry of tail-anchored proteins' (GET) pathway. This post-translational pathway uses transmembrane-domain selective cytosolic chaperones for targeting, and a dedicated membrane protein complex for insertion. The past decade has seen rapid progress towards defining the molecular basis of TA protein biogenesis by the GET pathway. Here we review the mechanisms underlying each step of the pathway, emphasizing recent structural work and highlighting key questions that await future studies.
摘要
许多尾部锚定（TA）膜蛋白通过“尾部锚定蛋白的引导进入”（GET）途径被靶向并插入内质网（ER）。这种翻译后途径使用跨膜域选择性胞质伴侣进行靶向，并使用专门的膜蛋白复合物进行插入。在过去的十年中，人们在通过 GET 途径定义 TA 蛋白生物发生的分子基础方面取得了快速进展。在这里，我们回顾了该途径每个步骤的机制，强调了最近的结构工作，并突出了有待未来研究解决的关键问题。
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