Üreyen O, Üstuner M A, Argon A, Özbilgin M, Egeli T, Ìlhan E, Vardar E, Ünek T, Yildirim M, Erkan N
Ìzmir Bozyaka Education and Research Hospital, Department of General Surgery, Ìzmir, Turkey.
Malays J Pathol. 2018 Aug;40(2):153-160.
The aim of this study was to investigate the efficacy of resveratrol and octreotide, agents that are used to prevent intra-abdominal adhesions in experimental models, in preventing intraperitoneal adhesions when used alone or in combination.
The study employed 28 young female Wistar albino rats weighing 250-300 grams. An experimental adhesion model was created in each rat using serosal abrasion and peritoneal excision. They were divided into four groups, each comprising seven rats: Group 1, adhesion induction only; Group 2, resveratrol administration only; Group 3, octreotide administration only; and Group 4, administration of resveratrol and octreotide combination. The rats were monitored under appropriate conditions for 14 days and then underwent laparotomy. Macroscopic intensity and extensiveness of adhesions and microscopic changes in the granulation tissue (cellular intensity, reticular and collagen fibers, capillaries, elastic and smooth muscle fibers, fibrosis) were evaluated and graded. Kruskal-Wallis and Mann-Whitney U-test were used in statistical analysis and the level of statistical significance was established as p <0.05.
There was no significant difference between the groups in terms of the intensity and extensiveness of macroscopic adhesions (p=0.377 and p=0.319). There was a statistically significant difference between the microscopic scores of the groups according to Zühlke's classification (p=0.026). The Bonferroni correction used to test for the differences revealed that the rats in Group 1 achieved significantly higher scores than the rats in Group 3 (p=0.016).
Octreotide showed higher efficiency compared to the control group in microscopic classification; however, the two agents were not superior to each other or their combination was not superior in preventing intra-abdominal adhesions.
本研究的目的是调查白藜芦醇和奥曲肽（这两种在实验模型中用于预防腹腔内粘连的药物）单独使用或联合使用时预防腹膜粘连的效果。
本研究使用了28只体重250 - 300克的年轻雌性Wistar白化大鼠。通过浆膜擦伤和腹膜切除在每只大鼠身上建立实验性粘连模型。将它们分为四组，每组七只大鼠：第1组，仅诱导粘连；第2组，仅给予白藜芦醇；第3组，仅给予奥曲肽；第4组，给予白藜芦醇和奥曲肽联合用药。在适当条件下对大鼠进行14天的监测，然后进行剖腹手术。评估并分级粘连的宏观强度和范围以及肉芽组织的微观变化（细胞强度、网状和胶原纤维、毛细血管、弹性和平滑肌纤维、纤维化）。统计分析采用Kruskal - Wallis和Mann - Whitney U检验，统计学显著性水平设定为p <0.05。
在宏观粘连的强度和范围方面，各组之间无显著差异（p = 0.377和p = 0.319）。根据祖尔克分类法，各组的微观评分存在统计学显著差异（p = 0.026）。用于检验差异的Bonferroni校正显示，第1组大鼠的得分显著高于第3组大鼠（p = 0.016）。
在微观分类中，奥曲肽显示出比对照组更高的效率；然而，这两种药物在预防腹腔内粘连方面并不优于彼此，它们的联合使用也不具有优势。
