[弱精子症与GRP78基因3'非翻译区多态性无关]

[Asthenozoospermia is not correlated with 3' UTR polymorphisms of the GRP78 gene].

作者信息

Qin Hai-Mei, Wang Rong, Pang Xiao-Xia, Wei Yu-Xia, Ling Zheng-Bao, Chen Xing-Hong, Wei Jing-Xi, Wang Jun-Li

机构信息

Laboratory of Reproductive Genetics, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China.

Graduate School of Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China.

出版信息

Zhonghua Nan Ke Xue. 2018 Jul;24(7):596-601.

PMID:30173441

Abstract

OBJECTIVE

To investigate the correlation of the single nucleotide polymorphisms (SNPs) rs12009, rs1140763 and rs16927997 in the 3'-untranslated region (3'UTR) of the glucose-regulated protein 78 (GRP78) gene with the risk of male asthenozoospermia (AZS).

METHODS

We included 400 AZS patients in the AZS group and another 400 fertile men as normal controls. Using the SNaPshot technique, we genotyped the rs12009, rs1140763 and rs16927997 polymorphisms in the 3'UTR of the GRP78 gene in all the male subjects and analyzed the association of the three SNPs with AZS.

RESULTS

The percentage of progressively motile sperm was significantly lower in the AZS group than in the normal controls (［20.09 ± 8.18］ % vs ［57.16 ± 13.45］ %, P <0.01). Three genotypes of CC, CT and TT and 2 alleles of C and T were found in rs12009 and rs1140763 of the GRP78 gene, and another three genotypes of GG, GA and AA and two alleles of G and A were observed in rs16927997. There were no statistically significant differences between the control and AZS groups in the frequencies of the C and T alleles in rs12009 (44.3% vs 47.3% and 55.7% vs 52.7%, P >0.05) or rs1140763 (50.0% vs 52.0% and 50.0% vs 48.0%, P >0.05) or those of the G and A alleles in rs16927997 (6.0% vs 4.4% and 94.0% vs 95.6%, P >0.05), nor in the genotypes and allele frequencies of the 3 polymorphisms (P >0.05). Furthermore, three haplotypes of C-C-A, T-C-G and T-T-A were observed in the male subjects but showed no evident correlation between the AZS and normal control groups.

CONCLUSIONS

The polymorphisms in the 3'UTR of the GRP78 gene are not correlated with the risk of male asthenozoospermia.

摘要

目的

探讨葡萄糖调节蛋白78（GRP78）基因3′非翻译区（3′UTR）单核苷酸多态性（SNP）rs12009、rs1140763和rs16927997与男性弱精子症（AZS）风险的相关性。

方法

将400例AZS患者纳入AZS组，另选400例有生育能力的男性作为正常对照组。采用SNaPshot技术对所有男性受试者GRP78基因3′UTR的rs12009、rs1140763和rs16927997多态性进行基因分型，并分析这3个SNP与AZS的相关性。

结果

AZS组精子前向运动百分比显著低于正常对照组（［20.09±8.18］% 对［57.16±13.45］%，P<0.01）。GRP78基因rs12009和rs1140763发现CC、CT和TT 3种基因型以及C和T 2个等位基因，rs16927997观察到GG、GA和AA 3种基因型以及G和A 2个等位基因。rs12009中C和T等位基因频率在对照组与AZS组之间（44.3%对47.3%以及55.7%对52.7%，P>0.05）、rs1140763中C和T等位基因频率在对照组与AZS组之间（50.0%对52.0%以及50.0%对48.0%，P>0.05）、rs16927997中G和A等位基因频率在对照组与AZS组之间（6.0%对4.4%以及94.0%对95.6%，P>0.05）均无统计学显著差异，3种多态性的基因型和等位基因频率在两组间也无统计学显著差异（P>0.05）。此外，男性受试者中观察到C-C-A、T-C-G和T-T-A 3种单倍型，但在AZS组与正常对照组之间未显示出明显相关性。