a Institute of Pharmaceutical Chemistry , University of Pécs , Pécs , Hungary.
b Department of Forensic Medicine , University of Pécs , Pécs , Hungary.
Free Radic Res. 2018 Sep;52(9):1040-1051. doi: 10.1080/10715762.2018.1517260. Epub 2018 Oct 12.
Fenton-reaction initiated in vitro oxidation and in vivo oxidative biotransformation of salicylic acid was investigated by HPLC-UV-Vis method. By means of the developed high performance liquid chromatography (HPLC) method salicylic acid, catechol, and all the possible monohydroxylated derivatives of salicylic acid can be separated. Fenton oxidations were performed in acidic medium (pH 3.0) with two reagent molar ratios: (1) salicylic acid: iron: hydrogen peroxide 1:3:1 and (2) 1:0.3:1. The incubation samples were analysed at different time points of the reactions. The biological effect of elevated reactive oxygen species concentration on the intestinal metabolism of salicylic acid was investigated by an experimental diabetic rat model. HPLC-MS analysis of the in vitro samples revealed presence of 2,3- and 2,5-dihydroxybenzoic acids. The results give evidence for nonenzyme catalysed intestinal hydroxylation of xenobiotics.
采用 HPLC-UV-Vis 法研究了芬顿反应引发的水杨酸体外氧化和体内氧化生物转化。通过开发的高效液相色谱 (HPLC) 方法,可以分离出水杨酸、儿茶酚和水杨酸的所有可能的单羟基化衍生物。芬顿氧化在酸性介质 (pH 3.0) 中进行,两种试剂摩尔比为:(1)水杨酸:铁:过氧化氢 1:3:1 和 (2) 1:0.3:1。在反应的不同时间点分析孵育样品。通过实验性糖尿病大鼠模型研究了升高的活性氧浓度对水杨酸肠道代谢的生物学影响。对体外样品的 HPLC-MS 分析表明存在 2,3-和 2,5-二羟基苯甲酸。结果为非酶催化的外源性物质肠内羟基化提供了证据。