Nappi A J, Vass E
Department of Biology, Loyola University Chicago, IL 60626, USA.
Biochim Biophys Acta. 1998 Mar 12;1380(1):55-63. doi: 10.1016/s0304-4165(97)00125-6.
The highly reactive and cytotoxic hydroxyl radical (OH) was found by electrochemical detection to be produced in reactions involving hydrogen peroxide (H2O2) and the nitric oxide (NO) donor diethylamine- NO complex. Using aromatic hydroxylation of salicylate as a specific indicator of OH, three salicylate hydroxylation products were identified; catechol, 2,3- and 2,5-dihydroxybenzoic acid. Four additional compounds were detected but not identified. The interactions of H2O2 and NO represent a biologically feasible reaction mechanism that can account for OH-induced damage in cellular environments where transition metal ions are unavailable for participation in the superoxide-mediated Fenton reaction. The ability of the NO/H2O2 complex to generate OH independently of iron or other transition metals provides a new focus for studies concerned with the origin of tissue-specific damage caused by oxygen-derived species.
通过电化学检测发现，在涉及过氧化氢（H2O2）和一氧化氮（NO）供体二乙胺 - NO络合物的反应中会产生高反应性和细胞毒性的羟基自由基（OH）。使用水杨酸的芳族羟基化作为OH的特异性指示剂，鉴定出三种水杨酸羟基化产物；儿茶酚、2,3 - 和2,5 - 二羟基苯甲酸。还检测到另外四种化合物但未鉴定。H2O2和NO的相互作用代表了一种生物学上可行的反应机制，这可以解释在过渡金属离子无法参与超氧化物介导的芬顿反应的细胞环境中OH诱导的损伤。NO/H2O2络合物独立于铁或其他过渡金属生成OH的能力为研究由氧衍生物种引起的组织特异性损伤的起源提供了新的重点。
