SomaGenics, Inc., Santa Cruz, California, USA.
Genome Biol. 2018 Sep 3;19(1):105. doi: 10.1186/s13059-018-1488-z.
The ability to accurately quantify all the microRNAs (miRNAs) in a sample is important for understanding miRNA biology and for development of new biomarkers and therapeutic targets. We develop a new method for preparing miRNA sequencing libraries, RealSeq®-AC, that involves ligating the miRNAs with a single adapter and circularizing the ligation products. When compared to other methods, RealSeq®-AC provides greatly reduced miRNA sequencing bias and allows the identification of the largest variety of miRNAs in biological samples. This reduced bias also allows robust quantification of miRNAs present in samples across a wide range of RNA input levels.
准确量化样品中所有 microRNAs (miRNAs) 的能力对于理解 miRNA 生物学以及开发新的生物标志物和治疗靶点非常重要。我们开发了一种新的 miRNA 测序文库制备方法，RealSeq®-AC，该方法涉及用单个接头连接 miRNAs 并环化连接产物。与其他方法相比，RealSeq®-AC 大大降低了 miRNA 测序偏差，并允许鉴定生物样品中最多样化的 miRNAs。这种降低的偏差还允许对 RNA 输入水平广泛变化的样品中存在的 miRNAs 进行稳健的定量。
