Institut des Sciences Vétérinaires, Université Blida1, 09000, Blida, Algeria.
Inserm U851, IFR 128, CNR des Staphylocoques, Université de Lyon1, 69008, Lyon, France; Centre de biologie Est, Hospices Civils de Lyon, 69500 Bron, France.
Vet Microbiol. 2018 Sep;223:173-180. doi: 10.1016/j.vetmic.2018.08.003. Epub 2018 Aug 3.
Staphylococcus aureus is a commensal and pathogen of both humans and bovines. While the epidemiology of both groups has been extensively studied individually, little is known about the potential zoonotic transfer from animal strains to human being and vice versa. To determine the S. aureus prevalence of bovine mastitis in Algeria and the zoonotic transfer of strains to human beings, mastitis milk samples were collected, and professionals in a close contact with bovines were nasal swabbed. S. aureus isolates were all characterized by methicillin resistance and spa-typing. DNA microarrays analysis was performed on a subset of strains in order to detect other virulence factors, including toxins, and to assign the isolates to theirs MLST clonal complexes. Overall, 116/222 (52.3%) cows suffered from mastitis, whose 38.8% (45/116) infected with S. aureus. Human nasal carriage was of 38% (49/129), with only 4 MRSA carriers (3.1%). A higher diversity of spa-types was observed in human (35/50) than in bovine (18/67) isolates, with a predominance of clonal complexes CC97 and CC22 in bovines. The typical animal clone CC97 was occasionally detected in human beings. Conversely, the CC22 S. aureus clone largely switched from humans to bovines. Our study highlights the potential dynamics of animal and human S. aureus strains in the farm environment in Algeria, which may represent a health threat in both populations.
金黄色葡萄球菌是人和牛的共生菌和病原体。虽然这两个群体的流行病学已经得到了广泛的研究，但对于从动物株向人类和反之的潜在人畜共患病转移知之甚少。为了确定阿尔及利亚奶牛乳腺炎中金黄色葡萄球菌的流行情况以及菌株向人类的人畜共患病转移，采集了乳腺炎奶样，并对与牛密切接触的专业人员进行了鼻腔拭子取样。金黄色葡萄球菌分离株均表现出耐甲氧西林和 spa 型特征。对部分菌株进行 DNA 微阵列分析，以检测其他毒力因子，包括毒素，并将分离株分配到它们的 MLST 克隆复合体。总体而言，222 头奶牛中有 116 头（52.3%）患有乳腺炎，其中 38.8%（45/116）感染了金黄色葡萄球菌。人类鼻腔携带率为 38%（49/129），仅 4 名 MRSA 携带者（3.1%）。在人类（35/50）中观察到比牛（18/67）分离株更高多样性的 spa 型，牛中以 CC97 和 CC22 克隆复合体为主导。偶尔会在人类中检测到典型的动物克隆 CC97。相反，CC22 金黄色葡萄球菌克隆从人类大量转移到牛。我们的研究强调了阿尔及利亚农场环境中动物和人类金黄色葡萄球菌菌株的潜在动态，这可能对这两个群体的健康构成威胁。
