Juwita Sartika, Indrawati Agustin, Damajanti Retno, Safika Safika, Mayasari Ni Luh Putu Ika
Department of Animal Disease and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia.
Vet World. 2022 Mar;15(3):558-564. doi: 10.14202/vetworld.2022.558-564. Epub 2022 Mar 10.
is a bacterium that causes several infectious diseases, including mastitis, endocarditis, and osteomyelitis, and poses a threat to human and animal health. This study aims to phenotypically and genetically identify from the isolates collected from humans, animals, environment, and Dangke products in the dairy farms of South Sulawesi Province, Indonesia, as well as to establish a genetic relationship among the isolated strains.
The total number of samples was 142, comprising 30 humans (skin swab), 58 animals (raw milk), 14 dairy products (Dangke), and 40 environmental samples (water). was phenotypically identified using the culture method, followed by Gram staining, catalase test, and coagulase test. Simultaneously, genotypic identification of was performed using the conventional polymerase chain reaction and sequencing methods. Sequencing data were analyzed using the MEGA X software by comparing BLAST National Center for Biotechnology Information databases.
The phenotypic methods revealed that 56/142 (39.4%) animal, human, and Dangke samples grew on culture, and 56/56 (100%) were Gram stain positive, 56/56 (100%) catalase-positive, and 23/56 (41.1%) coagulase positive. The genotypic method revealed that 32/56 (57.1%) samples amplified the gene. The phylogenetic analysis of 12 isolates revealed that they are all closely related and do not belong to distinct clades.
It indicates that isolates from animals (S30) are probably the same strain as human isolates (H2, H3, H4, and H5). The findings of this study can be used as information regarding the importance of preventing and controlling diseases caused by using a health approach involving the human, animal, and environmental sectors. This study was limited to the sequencing analysis of the gene.
[具体细菌名称未给出]是一种可引发多种传染病的细菌，包括乳腺炎、心内膜炎和骨髓炎，对人类和动物健康构成威胁。本研究旨在从印度尼西亚南苏拉威西省奶牛场采集的人类、动物、环境及当克产品的分离株中，对[具体细菌名称未给出]进行表型和基因鉴定，并确定分离出的[具体细菌名称未给出]菌株之间的遗传关系。
样本总数为142份，包括30份人类样本（皮肤拭子）、58份动物样本（生牛奶）、14份乳制品样本（当克）和40份环境样本（水）。采用培养方法对[具体细菌名称未给出]进行表型鉴定，随后进行革兰氏染色、过氧化氢酶试验和凝固酶试验。同时，使用常规聚合酶链反应和测序方法对[具体细菌名称未给出]进行基因鉴定。通过比较美国国立生物技术信息中心数据库，使用MEGA X软件对测序数据进行分析。
表型方法显示，56/142（39.4%）的动物、人类和当克样本在培养物上生长，56/56（100%）为革兰氏染色阳性，56/56（100%）过氧化氢酶阳性，23/56（41.1%）凝固酶阳性。基因方法显示，32/56（57.1%）的样本扩增出[具体基因名称未给出]基因。对12株分离株的系统发育分析表明，它们都密切相关，不属于不同的进化枝。
这表明来自动物的[具体细菌名称未给出]分离株（S30）可能与人类分离株（H2、H3、H4和H5）是同一菌株。本研究结果可作为通过涉及人类、动物和环境部门的健康方法预防和控制由[具体细菌名称未给出]引起的疾病的重要性的信息。本研究仅限于对[具体基因名称未给出]基因的测序分析。
