Jenkinson Carl, Taylor Angela E, Storbeck Karl-Heinz, Hewison Martin
Institute of Metabolism and Systems Research, The University of Birmingham, Birmingham B15 2TT, UK.
Department of Biochemistry, Stellenbosch University, Stellenbosch 7600, South Africa.
Data Brief. 2018 Aug 15;20:426-435. doi: 10.1016/j.dib.2018.08.027. eCollection 2018 Oct.
The data presented is related to the research article "Analysis of multiple vitamin D metabolites by ultra performance supercritical fluid chromatography-tandem mass spectrometry (UPSFC-MS/MS)" (Jenkinson et al., 2018) [1]. This article will include data obtained from method development, optimization and analysis of multiple vitamin D metabolites on an ultra performance supercritical fluid chromatography tandem-mass spectrometry (UPSFC-MS/MS). This includes chromatograms from column screening to confirm the most suitable column for analyte separation. Additionally, further chromatograms and figures compare separation and analyte signal strength during the optimization of other UPSFC parameters. Mass spectra will demonstrate the optimization of MS conditions for the UPSFC-MS/MS method. Chromatogram data from UHPLC vitamin D analysis is also presented in order to compare the separation and elution of vitamin D metabolites using UPSFC and UHPLC. This data will highlight the outputs that aid in method development and identifying the separation technique suited for vitamin D quantitation.
