用于检测伪狂犬病病毒的免疫过氧化物酶蚀斑染色法。
Immunoperoxidase plaque staining for the detection of pseudorabies virus.
作者信息
Afshar A, Dulac G C
出版信息
Can J Vet Res. 1986 Jan;50(1):118-9.
Abstract
A quantitative indirect immunoperoxidase plaque staining method was developed for the detection of pseudorabies virus infection in a pig kidney cell line (PK-15). The method is rapid and specific and foci of infection, represented by stained plaques, are easily counted by the unaided eye. Possible modification of this technique in a plaque reduction assay for the detection of antipseudorabies virus antibody is also discussed.
摘要
已开发出一种定量间接免疫过氧化物酶蚀斑染色法，用于检测猪肾细胞系（PK - 15）中的伪狂犬病病毒感染。该方法快速且特异，由染色蚀斑代表的感染灶可通过肉眼轻松计数。还讨论了该技术在蚀斑减少试验中用于检测抗伪狂犬病病毒抗体的可能改进。
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本文引用的文献
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Zentralbl Veterinarmed B. 1979 Apr;26(3):253-6. doi: 10.1111/j.1439-0450.1979.tb00813.x.
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