世界卫生组织关于使用单克隆抗体进行脊髓灰质炎病毒株型内鉴别诊断的合作研究。
WHO collaborative study on the use of monoclonal antibodies for the intratypic differentiation of poliovirus strains.
作者信息
Ferguson M, Magrath D I, Minor P D, Schild G C
出版信息
Bull World Health Organ. 1986;64(2):239-46.
An international collaborative study was carried out to identify monoclonal antibodies that could reliably discriminate between wild polioviruses and strains derived from Sabin vaccine viruses. For poliovirus types 2 and 3, monoclonal antibodies were identified that reacted specifically with type 2 or type 3 strains which gave T1-oligonucleotide maps similar to or indistinguishable from that of Sabin vaccine virus, thus indicating their vaccine origin. These monoclonal antibodies failed to react with strains which gave T1 maps unrelated to that of Sabin vaccine virus. However for type 1, five of the six antibodies examined in the study reacted only with strains with a T1 map indistinguishable from that of type 1 Sabin vaccine virus. In contrast, other monoclonal antibodies against poliovirus types 1, 2 and 3 reacted broadly within a serotype.
开展了一项国际合作研究，以鉴定能够可靠区分野生脊髓灰质炎病毒和源自萨宾疫苗病毒的毒株的单克隆抗体。对于2型和3型脊髓灰质炎病毒，鉴定出了与2型或3型毒株特异性反应的单克隆抗体，这些毒株的T1寡核苷酸图谱与萨宾疫苗病毒的图谱相似或无法区分，从而表明它们的疫苗来源。这些单克隆抗体与T1图谱与萨宾疫苗病毒无关的毒株不发生反应。然而，对于1型，该研究中检测的六种抗体中有五种仅与T1图谱与1型萨宾疫苗病毒无法区分的毒株发生反应。相比之下，其他针对1型、2型和3型脊髓灰质炎病毒的单克隆抗体在一个血清型内反应广泛。
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