Persu A, Combiescu M, Murg B, Aubert-Combiescu A
Cantacuzino Institute, Bucharest, Romania.
Roum Arch Microbiol Immunol. 1995 Jan-Jun;54(1-2):53-63.
By using a battery of poliovirus neutralizing monoclonal antibodies received from the Pasteur Institute, Paris, we carried out the study of the patterns of neutralization epitopes of more than 300 poliovirus strains isolated from cases of acute spinal paralysis and from healthy vaccinated children. We tried to find out the utility of this test for establishing the origin (wild or Sabin) of a poliovirus strain and the possible evolution of the pattern of neutralization epitopes. The study of the neutralization epitopes was carried out by neutralization index assay (NI). The neutralization index represents the difference between the titers of poliovirus strain determined in parallel in the absence, as well as in the presence of constant concentration (predetermined) for each monoclonal antibody. An epitope was considered to be present for a NI > or = 2 and absent for a NI < or = 1.5. For types 1 and 3 polioviruses, Sabin-like epitopes have never been found to be present simultaneously with wild type epitopes on the same virus particle. Therefore, examination of the epitope pattern of these strains is very useful for intratypic differentiation. On the other hand, in type 2 poliovirus strains, Sabin and wild type epitopes sometimes coexist, in various combinations, thus rendering the interpretation of the results rather difficult.
我们使用从巴黎巴斯德研究所获得的一组脊髓灰质炎病毒中和单克隆抗体，对从急性脊髓麻痹病例及健康接种儿童中分离出的300多株脊髓灰质炎病毒株的中和表位模式进行了研究。我们试图找出该检测对于确定脊髓灰质炎病毒株来源（野生型或萨宾株）以及中和表位模式可能演变的效用。中和表位的研究通过中和指数测定法（NI）进行。中和指数代表在不存在以及存在针对每种单克隆抗体的恒定浓度（预先确定）的情况下平行测定的脊髓灰质炎病毒株滴度之间的差异。当NI≥2时认为存在一个表位，当NI≤1.5时认为不存在。对于1型和3型脊髓灰质炎病毒，从未发现萨宾样表位与野生型表位在同一病毒颗粒上同时存在。因此，检查这些毒株的表位模式对于型内鉴别非常有用。另一方面，在2型脊髓灰质炎病毒株中，萨宾株和野生型表位有时以各种组合共存，从而使结果的解释相当困难。
