Ferguson M, Minor P D, Magrath D I, Schild G C
Dev Biol Stand. 1984;57:151-5.
Hybridoma cell lines secreting monoclonal antibodies to type 3 poliovirus have been prepared and their reactivity with infectious virus ('D' antigen), empty particles ('C' antigen) and isolated virus capsid proteins examined. Eight antibodies reacted with epitopes common to 'D' and 'C' antigen and all of these possessed high titres of neutralizing activity. However only 12 out of 19 antibodies which reacted exclusively with 'D' antigen neutralized virus infectivity and some of these reacted only with strains of virus with T1 oligonucleotide maps identical or similar to that of Sabin vaccine virus. These antibodies will be of value in identifying strains of virus derived from Sabin vaccine. None of the 20 monoclonal antibodies which neutralized type 3 poliovirus strains reacted in immunoblot experiments with isolated virus capsid proteins. However, 6 of the 24 antibodies which reacted only with non-infectious 'C' antigen bound to VP1 and VP3 and 3 of these antibodies also reacted with proteins of types 1 or 2 poliovirus. The lack of reactivity of neutralizing monoclonal antibodies with isolated viral proteins suggests that the antigenic properties of proteins are determined by their arrangement in the virus.
已制备出分泌抗3型脊髓灰质炎病毒单克隆抗体的杂交瘤细胞系，并检测了它们与感染性病毒（“D”抗原）、空病毒颗粒（“C”抗原）及分离出的病毒衣壳蛋白的反应性。8种抗体与“D”和“C”抗原共有的表位发生反应，并且所有这些抗体都具有高滴度的中和活性。然而，在仅与“D”抗原发生反应的19种抗体中，只有12种能中和病毒感染性，其中一些抗体仅与具有与Sabin疫苗病毒相同或相似的T1寡核苷酸图谱的病毒株发生反应。这些抗体在鉴定源自Sabin疫苗的病毒株方面将具有价值。在免疫印迹实验中，中和3型脊髓灰质炎病毒株的20种单克隆抗体均未与分离出的病毒衣壳蛋白发生反应。然而，仅与无感染性的“C”抗原发生反应的24种抗体中有6种与VP1和VP3结合，其中3种抗体还与1型或2型脊髓灰质炎病毒的蛋白发生反应。中和性单克隆抗体与分离出的病毒蛋白缺乏反应性表明，蛋白的抗原特性由其在病毒中的排列方式决定。
