Ventresca Miller Alicia, Fernandes Ricardo, Janzen Anneke, Nayak Ayushi, Swift Jillian, Zech Jana, Boivin Nicole, Roberts Patrick
Max Planck Institute for the Science of Human History;
Max Planck Institute for the Science of Human History; School of Archaeology, University of Oxford.
J Vis Exp. 2018 Aug 15(138):58002. doi: 10.3791/58002.
Stable carbon and oxygen isotope analysis of human and animal tooth enamel carbonate has been applied in paleodietary, paleoecological, and paleoenvironmental research from recent historical periods back to over 10 million years ago. Bulk approaches provide a representative sample for the period of enamel mineralization, while sequential samples within a tooth can track dietary and environmental changes during this period. While these methodologies have been widely applied and described in archaeology, ecology, and paleontology, there have been no explicit guidelines to aid in the selection of necessary lab equipment and to thoroughly describe detailed laboratory sampling and protocols. In this article, we document textually and visually, the entire process from sampling through pretreatment and diagenetic screening to make the methodology more widely available to researchers considering its application in a variety of laboratory settings.
对人类和动物牙釉质碳酸盐进行稳定碳和氧同位素分析，已被应用于从古近纪到一千多万年前的古饮食、古生态和古环境研究。总体方法可为牙釉质矿化期提供代表性样本，而牙齿内的连续样本可追踪该时期的饮食和环境变化。虽然这些方法已在考古学、生态学和古生物学中得到广泛应用和描述，但尚无明确的指导方针来帮助选择必要的实验室设备，也没有全面描述详细的实验室采样和方案。在本文中，我们通过文字和图像记录了从采样到预处理和成岩筛选的整个过程，以便考虑在各种实验室环境中应用该方法的研究人员更广泛地使用。
