Zondervan Robert L, Vorce Mitch, Servadio Nick, Hankenson Kurt D
College of Osteopathic Medicine, Michigan State University; Department of Orthopaedic Surgery, University of Michigan Medical School.
Lymann Briggs College, Michigan State University.
J Vis Exp. 2018 Aug 14(138):58186. doi: 10.3791/58186.
The reliable generation of consistent stabilized fractures in animal models is essential for understanding the biology of bone regeneration and developing therapeutics and devices. However, available injury models are plagued by inconsistency resulting in wasted animals and resources and imperfect data. To address this problem of fracture heterogeneity, the purpose of the method described herein is to optimize fracture generation parameters specific to each animal and yield a consistent fracture location and pattern. This protocol accounts for variations in bone size and morphology that may exist between mouse strains and can be adapted to generate consistent fractures in other species, such as rat. Additionally, a cost-effective, adjustable fracture apparatus is described. Compared to current stabilized fracture techniques, the optimization protocol and new fracture apparatus demonstrate increased consistency in stabilized fracture patterns and locations. Using optimized parameters specific to the sample type, the described protocol increases the precision of induced traumas, minimizing the fracture heterogeneity typically observed in closed-fracture generation procedures.
在动物模型中可靠地生成一致的稳定骨折对于理解骨再生生物学以及开发治疗方法和器械至关重要。然而，现有的损伤模型存在不一致性问题，导致动物和资源的浪费以及数据不完美。为了解决骨折异质性这一问题，本文所述方法的目的是优化特定于每只动物的骨折生成参数，并产生一致的骨折位置和模式。该方案考虑了小鼠品系之间可能存在的骨大小和形态差异，并且可以适用于在其他物种（如大鼠）中生成一致的骨折。此外，还描述了一种经济高效、可调节的骨折装置。与当前的稳定骨折技术相比，优化方案和新型骨折装置在稳定骨折模式和位置方面表现出更高的一致性。使用特定于样本类型的优化参数，所述方案提高了诱导创伤的精度，最大限度地减少了在闭合骨折生成过程中通常观察到的骨折异质性。
