黄颡鱼髓样分化因子 88 基因及其对多聚肌苷酸多聚胞苷酸和脂多糖刺激的分子特征。
A myeloid differentiation factor 88 gene from yellow catfish Pelteobagrus fulvidraco and its molecular characterization in response to polyriboinosinic polyribocytidylic acid and lipopolysaccharide challenge.
机构信息
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224051, People's Republic of China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, People's Republic of China; Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, People's Republic of China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224051, People's Republic of China.
出版信息
Int J Biol Macromol. 2018 Dec;120(Pt A):1080-1086. doi: 10.1016/j.ijbiomac.2018.08.189. Epub 2018 Aug 31.
Myeloid differentiation factor 88 (MyD88) is an adaptor protein of Toll-like receptor (TLR) signalling pathways that activates the innate immune system. Herein, MyD88 was identified in the economically important freshwater fish Pelteobagrus fulvidraco. The complete 2156 bp PfMyD88 cDNA includes a 147 bp 5'-untranslated region (UTR), a 1133 bp 3'-UTR, and an open reading frame (ORF) of 876 bp encoding a 291 residue protein containing Death and Toll/interleukin-1 receptor (TIR) domains. The deduced protein sequence shares 88.8%, 73.8% and 59.3% identity with orthologs in Ictalurus punctatus, Danio rerio and Homo sapiens, respectively. qRT-PCR revealed expression in all tested tissues, highest in trunk kidney, followed by spleen, and lowest in muscle. After challenge with lipopolysaccharide (LPS) or polyriboinosinic polyribocytidylic acid (Poly I:C), PfMyD88 expression was up-regulated in blood, liver, head kidney and spleen. Thus, PfMyD88 acts in innate immunity in P. fulvidraco.
髓样分化因子 88（MyD88）是 Toll 样受体（TLR）信号通路的衔接蛋白，可激活先天免疫系统。本文在经济重要的淡水鱼类黄颡鱼（Pelteobagrus fulvidraco）中鉴定到了 MyD88。PfMyD88 的完整 2156bp cDNA 包括 147bp 的 5'非翻译区（UTR）、1133bp 的 3'UTR 和 876bp 的开放阅读框（ORF），可编码一个由 291 个残基组成的蛋白质，含有死亡和 Toll/白细胞介素-1 受体（TIR）结构域。推导的蛋白序列与美洲鲶（Ictalurus punctatus）、斑马鱼（Danio rerio）和人类的同源物分别具有 88.8%、73.8%和 59.3%的同一性。qRT-PCR 显示 PfMyD88 在所有测试的组织中均有表达，在躯干肾脏中表达最高，其次是脾脏，在肌肉中表达最低。在脂多糖（LPS）或聚肌苷酸：聚胞苷酸（Poly I:C）刺激后，PfMyD88 在血液、肝脏、头肾和脾脏中的表达上调。因此，PfMyD88 在黄颡鱼的先天免疫中发挥作用。
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