补体因子 I (CFI) 基因介导黄颡鱼 Pelteobagrus fulvidraco 的先天免疫反应。
A complement factor I (CFI) gene mediates innate immune responses in yellow catfish Pelteobagrus fulvidraco.
机构信息
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetland, Yancheng Teachers University, Yancheng 224007, People's Republic of China; School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, People's Republic of China; College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China.
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetland, Yancheng Teachers University, Yancheng 224007, People's Republic of China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, College of Aquaculture and Life Science, Shanghai Ocean University, Shanghai 201306, People's Republic of China.
出版信息
Genomics. 2021 Jan;113(1 Pt 2):1257-1264. doi: 10.1016/j.ygeno.2020.09.034. Epub 2020 Sep 16.
This study isolated CFI gene from Pelteobagrus fulvidraco and named it PfCFI. The cDNA of PfCFI is 2374 bp long, including a 52 bp 5' untranslated sequence, a 222 bp 3' untranslated sequence, and an open reading frame (ORF) of 2100 bp encoding polypeptide consisting of 699 amino acids. Phylogenetic analysis revealed that the PfCFI was closely related to CFI of Ictalurus punctatus. Real-time quantitative reverse transcription-PCR (qRT-PCR) analysis indicate that there is the PfCFI gene which expressed in all the rest of tested tissues in varied levels, and mainly distributed in liver and least in heart. The reseachers induce the expressions level of PfCFI gene in liver, spleen, head kidney and blood at different points in time after challenged with lipopolysaccharide (LPS), and polyriboinosinic polyribocytidylic acid (poly I:C), respectively. Together these results suggested that CFI gene plays an important role in resistance to pathogens in yellow catfish immunity.
本研究从黄颡鱼（Pelteobagrus fulvidraco）中分离出 CFI 基因，并将其命名为 PfCFI。PfCFI 的 cDNA 长 2374bp，包括 52bp 的 5'非翻译区、222bp 的 3'非翻译区和一个编码由 699 个氨基酸组成的多肽的开放阅读框（ORF）。系统进化分析表明，PfCFI 与斑点叉尾鮰（Ictalurus punctatus）的 CFI 密切相关。实时定量反转录-PCR（qRT-PCR）分析表明，PfCFI 基因在所有检测组织中均有表达，且表达水平存在差异，主要在肝脏中表达，在心脏中表达最少。研究人员在黄颡鱼受到脂多糖（LPS）和聚肌胞苷酸（poly I:C）刺激后不同时间点分别诱导肝脏、脾脏、头肾和血液中 PfCFI 基因的表达水平。这些结果表明，CFI 基因在黄颡鱼免疫中对病原体的抗性中发挥重要作用。
Zotero 插件