College of Marine Life Science, Ocean University of China, Qingdao, China.
Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fisheries Research, Qingdao, China.
Fish Shellfish Immunol. 2018 Nov;82:573-578. doi: 10.1016/j.fsi.2018.08.058. Epub 2018 Aug 31.
Receptor interacting protein kinase 3 (RIP3) has emerged as an essential molecular regulator in human inflammation with accumulating evidence in vertebrates. However, the structure and functions of RIP3 in teleosts remains elusive. Here we identified one RIP3 homologue from half-smooth tongue sole Cynoglossus semilaevis, designated CsRIP3. The open reading frame (ORF) of CsRIP3 contained 1491 nucleotides and encoded a protein with 496 amino acids (Mw = 55.44 kDa). CsRIP3 expression was detected in various tissues in half-smooth tongue soles. CsRIP3 expression was up-regulated after pathogens i.e. Vibrio and poly (i:c) treatment in vivo, indicating its possible role in teleost immune response. Further analysis using human cells demonstrated that CsRIP3 exhibited highly conserved pro-apoptotic and pro-necroptotic functions in comparison with human RIP3. In conclusion, for the first time we reported the homologous structure and functions of CsRIP3 in inflammatory cell death, which provides novel perspectives on fish immunity studies in future.
受体相互作用蛋白激酶 3（RIP3）已成为脊椎动物中人类炎症的重要分子调节剂，有越来越多的证据表明。然而，硬骨鱼中 RIP3 的结构和功能仍不清楚。本研究从半滑舌鳎 Cynoglossus semilaevis 中鉴定到一个 RIP3 同源物，命名为 CsRIP3。CsRIP3 的开放阅读框（ORF）包含 1491 个核苷酸，编码一个含有 496 个氨基酸的蛋白质（Mw=55.44 kDa）。在半滑舌鳎的各种组织中都检测到 CsRIP3 的表达。CsRIP3 的表达在体内受到病原体（如弧菌和 poly（i：c））处理后上调，表明其可能在硬骨鱼免疫反应中发挥作用。进一步用人细胞进行分析表明，与人类 RIP3 相比，CsRIP3 具有高度保守的促凋亡和促坏死功能。总之，本研究首次报道了 CsRIP3 在炎症细胞死亡中的同源结构和功能，为今后鱼类免疫研究提供了新的视角。
