Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science and Fisheries, Huaihai Institute of Technology, Lianyungang, 222005, China; Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, China.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Fish Shellfish Immunol. 2018 Jun;77:392-401. doi: 10.1016/j.fsi.2018.04.007. Epub 2018 Apr 7.
In mammals, microtubule-dependent trafficking could participate the immune response, where the motor proteins are suggested to play an important role in this process, while the related study in fish was rare. In this study, dctn5, a subunit of dyactin complex for docking motor protein, was obtained by previous immune QTL screening. The full-length cDNAs of two dctn5 transcript variants were cloned and identified (named dctn5_tv1 and dctn5_tv2, respectively). Tissue distribution showed that dctn5_tv1 was widely distributed and high transcription was observed in immune tissue (skin), while dctn5_tv2 was predominantly detected in gonad and very low in other tissues. Time-course expression analysis revealed that dctn5_tv1 could be up-regulated in gill, intestine, skin, spleen, and kidney after Vibrio harveyi challenge. Moreover, recombinant Dctn5_tv1 exhibited high antimicrobial activity against Escherichia coli and Streptococcus agalactiae due to binding to bacteria cells. Taken together, these data suggest Dctn5_tv1 is involved in immune response of bacterial invasion in Chinese tongue sole.
在哺乳动物中，微管依赖性运输可能参与免疫反应，其中运动蛋白被认为在这个过程中发挥重要作用，而鱼类的相关研究则很少。在这项研究中，通过之前的免疫 QTL 筛选获得了肌动蛋白复合物的一个亚基 dctn5，用于停靠运动蛋白。克隆并鉴定了两种 dctn5 转录变体的全长 cDNA（分别命名为 dctn5_tv1 和 dctn5_tv2）。组织分布表明 dctn5_tv1 广泛分布，在免疫组织（皮肤）中转录水平较高，而 dctn5_tv2 主要在性腺中检测到，在其他组织中很少。时间过程表达分析显示，在哈维弧菌攻毒后，dctn5_tv1 可在鲆鱼的鳃、肠、皮肤、脾和肾中被诱导上调。此外，由于与细菌细胞结合，重组 Dctn5_tv1 对大肠杆菌和无乳链球菌表现出高的抗菌活性。综上所述，这些数据表明 Dctn5_tv1 参与了中国牙鲆抵抗细菌入侵的免疫反应。
