比较网络分析揭示了在不同水力负荷下渗滤液回灌去除抗生素抗性基因的机制。
Comparative network analysis revealing the mechanisms of antibiotic resistance genes removal by leachate recirculation under different hydraulic loadings.
机构信息
Key Laboratory for Urban and Ecological Restoration of Shanghai, School of Ecology and Environmental Sciences, East China Normal University, Shanghai 200241, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
Key Laboratory for Urban and Ecological Restoration of Shanghai, School of Ecology and Environmental Sciences, East China Normal University, Shanghai 200241, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
出版信息
Sci Total Environ. 2019 Feb 1;649:318-326. doi: 10.1016/j.scitotenv.2018.08.361. Epub 2018 Aug 27.
The wide dissemination of antibiotic resistance is a pervasive global health threat, and landfill leachate has been an important hotspot of antibiotic resistance genes (ARGs). This study aimed to investigate the removal performance and mechanism of ARGs from leachate under different hydraulic loadings. ARGs removal efficiencies were dependent on hydraulic loadings and ARGs types other than operating time, and reactors operated with hydraulic loadings of 25 and 50 L·m·d exhibited greater removal potential than 100 L·m·d. ARGs removal patterns varied from different subtypes, for genes sul2, tetQ, aadA1 and bla were eliminated from both leachate and refuse, and tetM, ermB, mefA, and strB were removed from leachate but enriched in refuse. Under different hydraulic loadings, bacterial communities shift shaped ARGs fates in leachate, but refuse had more stable antibiotic resistome and community structure. The topology comparison analysis of co-occurrence network suggested a closer hosting relationship between ARGs and genera in refuse than leachate. Furthermore, taxonomic category of host bacteria other than diversity of host genera determined the ARGs removal, and the ARGs harbored in phyla Cyanobacteria, Tenericutes and Acidobacteria were more likely to be removed. These findings can potentially foster the understanding of ARGs removal mechanism in biological treatment processes under different operating conditions.
抗生素耐药性的广泛传播是一个普遍存在的全球健康威胁，而垃圾填埋场渗滤液一直是抗生素耐药基因（ARGs）的一个重要热点。本研究旨在探讨不同水力负荷下渗滤液中 ARGs 的去除性能和机制。ARGs 的去除效率取决于水力负荷和 ARGs 类型，而与运行时间无关，水力负荷为 25 和 50 L·m·d 的反应器比 100 L·m·d 的反应器具有更大的去除潜力。ARGs 的去除模式因不同的亚型而异，对于 sul2、tetQ、aadA1 和 bla 基因，它们既从渗滤液中又从垃圾中被去除，而 tetM、ermB、mefA 和 strB 则从渗滤液中被去除，但在垃圾中富集。在不同的水力负荷下，细菌群落的变化影响了渗滤液中 ARGs 的命运，但垃圾具有更稳定的抗生素抗性组和群落结构。共现网络拓扑比较分析表明，ARGs 与垃圾中的属之间存在更密切的宿主关系，而不是渗滤液。此外，宿主细菌的分类类别而非宿主属的多样性决定了 ARGs 的去除，而在蓝藻门、无壁菌门和酸杆菌门中携带的 ARGs 更有可能被去除。这些发现可能有助于理解在不同运行条件下生物处理过程中 ARGs 的去除机制。
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