1 Institute of Dentistry, University of Turku, Finland.
2 Oral Health Care, Welfare Division, Finland.
Innate Immun. 2018 Oct;24(7):439-447. doi: 10.1177/1753425918796207. Epub 2018 Sep 3.
Genetic factors play a role in periodontitis. Here we examined whether the risk haplotype of MHC class III region BAT1-NFKBIL1-LTA and lymphotoxin-α polymorphisms associate with salivary biomarkers of periodontal disease. A total of 455 individuals with detailed clinical and radiographic periodontal health data were included in the study. A 610 K genotyping chip and a Sequenom platform were used in genotyping analyses. Phospholipid transfer protein activity, concentrations of lymphotoxin-α, IL-8 and myeloperoxidase, and a cumulative risk score (combining Porphyromonas gingivalis, IL-1β and matrix metalloproteinase-8) were examined in saliva samples. Elevated IL-8 and myeloperoxidase concentrations and cumulative risk scores associated with advanced tooth loss, deepened periodontal pockets and signs of periodontal inflammation. In multiple logistic regression models adjusted for periodontal parameters and risk factors, myeloperoxidase concentration (odds ratio (OR); 1.37, P = 0.007) associated with increased odds for having the risk haplotype and lymphotoxin-α concentration with its genetic variants rs2857708, rs2009658 and rs2844482. In conclusion, salivary levels of IL-8, myeloperoxidase and cumulative risk scores associate with periodontal inflammation and tissue destruction, while those of myeloperoxidase and lymphotoxin-α associate with genetic factors as well.
遗传因素在牙周炎中起作用。在这里，我们研究了 MHC Ⅲ类区域 BAT1-NFKBIL1-LTA 和淋巴毒素-α多态性的风险单倍型是否与牙周病的唾液生物标志物相关。共有 455 名个体具有详细的临床和影像学牙周健康数据被纳入研究。使用 610K 基因分型芯片和 Sequenom 平台进行基因分型分析。在唾液样本中检测了磷脂转移蛋白活性、淋巴毒素-α、IL-8 和髓过氧化物酶的浓度以及累积风险评分（结合牙龈卟啉单胞菌、IL-1β 和基质金属蛋白酶-8）。升高的 IL-8 和髓过氧化物酶浓度以及累积风险评分与牙齿缺失加重、牙周袋加深和牙周炎炎症迹象相关。在调整了牙周参数和危险因素的多元逻辑回归模型中，髓过氧化物酶浓度（比值比（OR）；1.37，P=0.007）与具有风险单倍型的可能性增加相关，淋巴毒素-α浓度与遗传变异 rs2857708、rs2009658 和 rs2844482 相关。总之，IL-8、髓过氧化物酶和累积风险评分与牙周炎炎症和组织破坏相关，而髓过氧化物酶和淋巴毒素-α与遗传因素相关。
