PEX Biotechnologie Chimie & Biologie, Institut de Recherches Servier, Croissy sur Seine, France.
Institut des Neurosciences Cellulaires et Intégratives, Strasbourg, France.
Sci Rep. 2018 Sep 3;8(1):13167. doi: 10.1038/s41598-018-31506-2.
Hibernation is an exceptional physiological response to a hostile environment, characterized by a seasonal period of torpor cycles involving dramatic reductions of body temperature and metabolism, and arousal back to normothermia. As the mechanisms regulating hibernation are still poorly understood, here we analysed the expression of genes involved in energy homeostasis, torpor regulation, and daily or seasonal timing using digital droplet PCR in various central and peripheral tissues sampled at different stages of torpor/arousal cycles in the European hamster. During torpor, the hypothalamus exhibited strongly down-regulated gene expression, suggesting that hypothalamic functions were reduced during this period of low metabolic activity. During both torpor and arousal, many structures (notably the brown adipose tissue) exhibited altered expression of deiodinases, potentially leading to reduced tissular triiodothyronine availability. During the arousal phase, all analysed tissues showed increased expression of the core clock genes Per1 and Per2. Overall, our data indicated that the hypothalamus and brown adipose tissue were the tissues most affected during the torpor/arousal cycle, and that clock genes may play critical roles in resetting the body's clocks at the beginning of the active period.
冬眠是一种对恶劣环境的特殊生理反应，其特征是周期性的蛰伏期，包括体温和代谢的剧烈下降，以及恢复到正常体温。由于调节冬眠的机制仍不清楚，在这里我们使用数字液滴 PCR 分析了在欧洲仓鼠的蛰伏/苏醒周期的不同阶段采集的各种中枢和外周组织中与能量稳态、蛰伏调节和日常或季节性时间有关的基因的表达。在蛰伏期间，下丘脑表现出强烈下调的基因表达，这表明在下丘脑的代谢活动较低的时期，其功能受到了抑制。在蛰伏和苏醒期间，许多结构（特别是棕色脂肪组织）表现出脱碘酶表达的改变，可能导致组织中三碘甲状腺原氨酸的可用性降低。在苏醒期，所有分析的组织都表现出核心时钟基因 Per1 和 Per2 的表达增加。总的来说，我们的数据表明，在蛰伏/苏醒周期中，下丘脑和棕色脂肪组织是受影响最大的组织，而时钟基因可能在活动期开始时对重置身体的生物钟起着关键作用。
