Kisliuk R L, Tattersall M H, Gaumont Y, Pastore E J, Brown B
Cancer Treat Rep. 1977 Jul;61(4):647-50.
An ip injection of a Lactobacillus casei dihydrofolate reductase preparation into rats and mice given a single lethal dose of methotrexate (MTX) caused a marked lowering of free MTX in the blood. Alternatives to citrovorum factor as agents for reversing MTX toxicity were explored in mice. dl, L-5-Methyltetrahydrofolate, dl,L-5,10-methylenetetrahydrofolate, l,L-5,10-methylenetetrahydrofolate, and dihydrofolate were also effective MTX antagonists; d,L-5,10-methylenetetrahydrofolate was inert.
向给予单次致死剂量甲氨蝶呤（MTX）的大鼠和小鼠腹腔注射干酪乳杆菌二氢叶酸还原酶制剂，可使血液中游离MTX显著降低。在小鼠中探索了用除亚叶酸之外的其他物质作为逆转MTX毒性的药物。dl，L - 5 - 甲基四氢叶酸、dl，L - 5，10 - 亚甲基四氢叶酸、l，L - 5，10 - 亚甲基四氢叶酸和二氢叶酸也是有效的MTX拮抗剂；d，L - 5，10 - 亚甲基四氢叶酸无活性。
