Rafalski J A
Gene. 1986;43(3):221-9. doi: 10.1016/0378-1119(86)90210-6.
We have cloned and sequenced two linked members of the wheat (Triticum aestivum) gliadin multigene family. One gene encodes a gamma-gliadin which is 292 amino acids (aa) long. S1 mapping indicates that this gene is transcriptionally active. The second gene, which is only marginally active by S1 mapping, is closely related to the first gene. Moreover, it is probably incapable of encoding a full-length gamma-gliadin due to the presence of two premature in-frame stop codons. Neither gene contains introns. Nucleotide sequences of the two genes show the presence of characteristic repeats which have a derived aa consensus sequence Pro-Gln-Gln-Pro-Gln-Gln-Pro-Phe-Pro-Gln. A comparison of the promoter regions of these gamma-gliadin genes with those of the alpha-gliadin genes shows the presence of a highly conserved region that could be involved in tissue specific and developmental regulation.
我们克隆并测序了小麦（普通小麦）醇溶蛋白多基因家族的两个连锁成员。一个基因编码一种由292个氨基酸组成的γ-醇溶蛋白。S1作图表明该基因具有转录活性。第二个基因通过S1作图显示活性微弱，与第一个基因密切相关。此外，由于存在两个框内过早终止密码子，它可能无法编码全长γ-醇溶蛋白。两个基因均不含内含子。这两个基因的核苷酸序列显示存在特征性重复序列，其推导的氨基酸共有序列为Pro-Gln-Gln-Pro-Gln-Gln-Pro-Phe-Pro-Gln。将这些γ-醇溶蛋白基因的启动子区域与α-醇溶蛋白基因的启动子区域进行比较发现，存在一个高度保守的区域，该区域可能参与组织特异性和发育调控。
