一种建立永久性生长淋巴母细胞系的常规方法。
A routine method for the establishment of permanent growing lymphoblastoid cell lines.
作者信息
Neitzel H
出版信息
Hum Genet. 1986 Aug;73(4):320-6. doi: 10.1007/BF00279094.
PMID:3017841
Abstract
Permanent lymphoblastoid cell lines are of great practical value in human clinical and experimental genetics. A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Epstein-Barr virus and the immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation system is briefly summarized.
摘要
永久性淋巴母细胞系在人类临床和实验遗传学中具有重大实用价值。本文给出了一份详细的常规操作方案，介绍了如何利用爱泼斯坦-巴尔病毒和免疫抑制剂环孢菌素A从外周血建立淋巴母细胞系。此外，还简要概述了该转化系统的生物学基础。
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