通过白细胞游走抑制和阻断抗体检测爱泼斯坦-巴尔病毒转化的非生产性细胞中的爱泼斯坦-巴尔病毒相关膜抗原。
Detection of an Epstein-Barr virus-associated membrane antigen in Epstein-Barr virus-transformed nonproducer cells by leukocyte migration inhibition and blocking antibody.
作者信息
Szigeti R, Sulitzeanu D, Henle G, Henle W, Klein G
出版信息
Proc Natl Acad Sci U S A. 1984 Jul;81(13):4178-82. doi: 10.1073/pnas.81.13.4178.
Abstract
Soluble membrane fractions derived from Raji cells trigger lymphocytes of Epstein-Barr virus (EBV)-seropositive, but not EBV-seronegative, individuals to release a lymphokine that inhibits leukocyte migration. The reaction can be blocked by the sera of patients with EBV-DNA-carrying tumors, Burkitt lymphoma, or nasopharyngeal carcinoma. Absorption of these sera with EBV-positive, but not EBV-negative, cells abrogates their blocking activity. These findings suggest that the antigen responsible for the leukocyte migration inhibition reaction is an EBV-encoded or an EBV-induced membrane component. The antigen is not identical with EBV-associated nuclear antigen or any other known antibody-detected EBV antigen.
摘要
来自拉吉细胞的可溶性膜组分可触发爱泼斯坦-巴尔病毒（EBV）血清反应阳性个体的淋巴细胞释放一种抑制白细胞迁移的淋巴因子，但不会触发EBV血清反应阴性个体的淋巴细胞。EBV-DNA携带肿瘤、伯基特淋巴瘤或鼻咽癌患者的血清可阻断该反应。用EBV阳性细胞而非EBV阴性细胞吸收这些血清可消除其阻断活性。这些发现表明，负责白细胞迁移抑制反应的抗原是一种EBV编码或EBV诱导的膜成分。该抗原与EBV相关核抗原或任何其他已知的可通过抗体检测到的EBV抗原不同。
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Detection of an Epstein-Barr virus-associated membrane antigen in Epstein-Barr virus-transformed nonproducer cells by leukocyte migration inhibition and blocking antibody.通过白细胞游走抑制和阻断抗体检测爱泼斯坦-巴尔病毒转化的非生产性细胞中的爱泼斯坦-巴尔病毒相关膜抗原。
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