人心脏细胞色素c氧化酶的分离与特性研究
Isolation and characterization of human heart cytochrome c oxidase.
作者信息
Kuhn-Nentwig L, Kadenbach B
出版信息
J Bioenerg Biomembr. 1986 Aug;18(4):307-14. doi: 10.1007/BF00743051.
PMID:3017929
Abstract
Cytochrome c oxidase was isolated from human hearts and separated by SDS gel electrophoresis. The identity of polypeptide bands with known subunits was demonstrated by immunoblotting with monospecific antisera to rat liver cytochrome c oxidase subunits. The polarographically determined kinetics of cytochrome c oxidation were similar to those reported for the bovine heart enzyme.
摘要
细胞色素c氧化酶从人心脏中分离出来，并通过SDS凝胶电泳进行分离。用大鼠肝脏细胞色素c氧化酶亚基的单特异性抗血清进行免疫印迹，证实了具有已知亚基的多肽条带的身份。极谱法测定的细胞色素c氧化动力学与报道的牛心脏酶的动力学相似。
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