Department of Biology , Trent University , Peterborough , Ontario K9L 0G2 , Canada.
International Institute for Sustainable Development , Winnipeg , Manitoba R3B 0T4 , Canada.
Environ Sci Technol. 2018 Oct 2;52(19):11114-11122. doi: 10.1021/acs.est.8b03146. Epub 2018 Sep 17.
A total of 15 kg of silver nanoparticles (AgNPs) was added continuously over two ice-free field seasons to a boreal lake (i.e., Lake 222) at the IISD Experimental Lakes Area in Canada. We monitored the accumulation of silver (Ag) in the tissues of yellow perch ( Perca flavescens) and northern pike ( Esox lucius) exposed to the AgNPs under environmentally relevant conditions. The greatest accumulation was observed in the liver tissues of pike, and a single pike sampled in the second year of additions had the highest concentration observed in liver of 5.1 micrograms per gram of wet weight. However, the Ag concentrations in gill and muscle tissue of both pike and perch did not exceed 0.35 micrograms per gram of wet weight. Following additions of AgNP, the Ag residues in fish tissues declined, with a half-life of Ag in pike liver of 119 days. Monitoring using passive sampling devices and single-particle inductively coupled plasma mass spectrometry during the AgNP addition phase confirmed that Ag nanoparticles were present in the water column and that estimated mean concentrations of Ag increased over time to a maximum of 11.5 μg/L. These data indicate that both a forage fish and a piscivorous fish accumulated Ag in a natural lake ecosystem dosed with AgNPs, leading to Ag concentrations in some tissues of the piscivorous species that were 3 orders of magnitude greater than the concentrations in the water.
在加拿大国际可持续发展研究所实验湖区，连续两个无冰季向一个北方湖泊（即 222 号湖）中添加了总计 15 公斤的银纳米颗粒（AgNPs）。我们监测了在环境相关条件下暴露于 AgNPs 的黄鲈（ Perca flavescens）和北方梭鲈（ Esox lucius）组织中银（Ag）的积累情况。在鱼的肝脏组织中观察到最大的积累，在添加的第二年中采样的一条梭鲈的肝脏中观察到最高浓度为 5.1 微克/克湿重。然而，鱼的鳃和肌肉组织中的 Ag 浓度均未超过 0.35 微克/克湿重。AgNP 添加后，鱼组织中的 Ag 残留物减少，Ag 在梭鲈肝脏中的半衰期为 119 天。在 AgNP 添加阶段使用被动采样装置和单颗粒电感耦合等离子体质谱监测证实，Ag 纳米颗粒存在于水柱中，Ag 的估计平均浓度随时间增加，最高达到 11.5μg/L。这些数据表明，在自然湖泊生态系统中，AgNPs 的添加导致了一种饲料鱼和一种肉食性鱼积累了 Ag，导致某些肉食性鱼类组织中的 Ag 浓度比水中的浓度高 3 个数量级。
