Department of Biology, Lakehead University, Thunder Bay, ON, Canada.
IISD Experimental Lakes Area Inc., Winnipeg, MB, Canada.
Arch Environ Contam Toxicol. 2020 Oct;79(3):283-297. doi: 10.1007/s00244-020-00764-5. Epub 2020 Oct 15.
Silver nanoparticles (AgNP) are widely used as antibacterial agents in both commercial products and for industrial applications. As such, AgNP has a high potential for release into freshwater environments. As part of a whole-lake ecosystem experiment to examine the impacts of AgNP exposure at low µg/L concentrations over multiple years, we evaluated biological responses in Yellow Perch (Perca flavescens) before, during, and after AgNP additions to a freshwater lake. Yellow Perch were monitored for responses to in situ AgNP additions at the cellular (suite of biomarkers), individual (growth, prey consumption, and metabolism), and population (abundance and gross prey consumption) scales. At the cellular level, several biomarkers of oxidative stress in liver tissues revealed down-regulation, including decreased mRNA levels of catalase and glutathione peroxidase in Yellow Perch collected during AgNP exposure, and elevated ratios of reduced to oxidized glutathione. At the individual level, Yellow Perch bioenergetic models revealed that prey consumption and total metabolism significantly declined during AgNP additions and remained depressed one year after AgNP addition. At the population level, Yellow Perch densities and gross prey consumption declined after AgNP was added to the lake. Together, these results reveal a holistic assessment of the negative impacts of chronic exposure to environmentally relevant AgNP concentrations (i.e., µg/L) on Yellow Perch at cellular, individual, and population levels.
银纳米颗粒（AgNP）作为商业产品和工业应用中的抗菌剂被广泛使用。因此，AgNP 具有很高的释放到淡水环境中的潜力。作为一项全湖生态系统实验的一部分，该实验旨在研究在多年时间内以低微克/升浓度暴露于 AgNP 对环境的影响，我们评估了黄鲈（Perca flavescens）在 AgNP 被添加到淡水湖中之前、期间和之后的生物反应。黄鲈被监测以了解对原位 AgNP 添加的反应，包括在细胞（一整套生物标志物）、个体（生长、猎物消耗和新陈代谢）和种群（丰度和总猎物消耗）水平上的反应。在细胞水平上，肝脏组织中几种氧化应激生物标志物的下调，包括在 AgNP 暴露期间收集的黄鲈中过氧化氢酶和谷胱甘肽过氧化物酶的 mRNA 水平降低，以及还原型与氧化型谷胱甘肽的比值升高。在个体水平上，黄鲈生物能量学模型表明，在 AgNP 添加期间，猎物消耗和总新陈代谢显著下降，并且在 AgNP 添加一年后仍处于抑制状态。在种群水平上，在向湖中添加 AgNP 后，黄鲈的密度和总猎物消耗下降。总的来说，这些结果全面评估了慢性暴露于环境相关浓度的 AgNP（即微克/升）对黄鲈在细胞、个体和种群水平上的负面影响。
