Niedbalski Peter, Kiswandhi Andhika, Parish Christopher, Wang Qing, Khashami Fatemeh, Lumata Lloyd
Department of Physics , The University of Texas at Dallas , 800 West Campbell Road , Richardson , Texas 75080 , United States.
J Phys Chem Lett. 2018 Sep 20;9(18):5481-5489. doi: 10.1021/acs.jpclett.8b01687. Epub 2018 Sep 10.
Dynamic nuclear polarization (DNP) via the dissolution method is one of the most successful methods for alleviating the inherently low Boltzmann-dictated sensitivity in nuclear magnetic resonance (NMR) spectroscopy. This emerging technology has already begun to positively impact chemical and metabolic research by providing the much-needed enhancement of the liquid-state NMR signals of insensitive nuclei such as C by several thousand-fold. In this Perspective, we present our viewpoints regarding the key elements needed to maximize the NMR signal enhancements in dissolution DNP, from the very core of the DNP process at cryogenic temperatures, DNP instrumental conditions, and chemical tuning in sample preparation to current developments in minimizing hyperpolarization losses during the dissolution transfer process. The optimization steps discussed herein could potentially provide important experimental and theoretical considerations in harnessing the best possible sensitivity gains in NMR spectroscopy as afforded by optimized dissolution DNP technology.
通过溶解法实现的动态核极化（DNP）是缓解核磁共振（NMR）光谱中固有的由玻尔兹曼分布决定的低灵敏度的最成功方法之一。这项新兴技术已开始通过将诸如碳等不灵敏核的液态NMR信号增强数千倍，对化学和代谢研究产生积极影响。在这篇展望文章中，我们阐述了关于在溶解DNP中最大化NMR信号增强所需关键要素的观点，从低温下DNP过程的核心、DNP仪器条件、样品制备中的化学调控，到溶解转移过程中最小化超极化损失的当前进展。本文讨论的优化步骤可能为利用优化的溶解DNP技术在NMR光谱中获得最佳灵敏度增益提供重要的实验和理论考量。
