Bodó Kornélia, Boros Ákos, Rumpler Éva, Molnár László, Böröcz Katalin, Németh Péter, Engelmann Péter
Department of Immunology and Biotechnology, Clinical Center, Medical School, University of Pécs, Hungary.
Regional Laboratory of Virology, ANTSZ, Baranya County Institute of State Public Health Service, Pécs, Hungary.
Dev Comp Immunol. 2019 Jan;90:41-46. doi: 10.1016/j.dci.2018.09.001. Epub 2018 Sep 1.
Lumbricin and its orthologue antimicrobial peptides were typically isolated from annelids. In this report, mRNA for lumbricin and -serendipitously- a novel lumbricin-related mRNA sequence were identified in Eisenia andrei earthworms. The determined mRNA sequences of E. andrei lumbricin and lumbricin-related peptide consist of 477 and 575 nucleotides. The precursors of proline-rich E. andrei lumbricin and the related peptide contain 63 and 59 amino acids, respectively. Phylogenetic analysis indicated close relationship with other annelid lumbricins. Highest expression of both mRNAs appeared in the proximal part of the intestine (pharynx, gizzard), while other tested organs had moderate (body wall, midgut, ovary, metanephridium, seminal vesicles, ventral nerve cord) or low (coelomocytes) levels. During ontogenesis their expression revealed continuous increase in embryos. Following 48 h of in vivo Gram-positive bacteria challenge both mRNAs were significantly elevated in coelomocytes, while Gram-negative bacteria or zymosan stimulation had no detectable effects.
蚓激酶及其同源抗菌肽通常是从环节动物中分离得到的。在本报告中，在安德爱胜蚓中鉴定出了蚓激酶的信使核糖核酸（mRNA），并且意外地发现了一种与蚓激酶相关的新mRNA序列。已确定的安德爱胜蚓蚓激酶和蚓激酶相关肽的mRNA序列分别由477个和575个核苷酸组成。富含脯氨酸的安德爱胜蚓蚓激酶及其相关肽的前体分别包含63个和59个氨基酸。系统发育分析表明，它们与其他环节动物的蚓激酶关系密切。两种mRNA在肠道近端（咽、砂囊）的表达最高，而其他检测器官的表达水平中等（体壁、中肠、卵巢、后肾、精囊、腹神经索）或较低（体腔细胞）。在个体发育过程中，它们在胚胎中的表达呈持续增加趋势。在体内用革兰氏阳性菌攻击48小时后，两种mRNA在体腔细胞中的表达均显著升高，而革兰氏阴性菌或酵母聚糖刺激则未产生可检测到的影响。
