Thayer School of Engineering at Dartmouth, 14 Engineering Drive, Hanover, NH 03755, United States.
Geisel School of Medicine at Dartmouth, 1 Rope Ferry Road, Hanover, NH 03755, United States.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Oct 1;1096:208-213. doi: 10.1016/j.jchromb.2018.08.032. Epub 2018 Aug 29.
In this study, the volatile molecule profile of Streptococcus pneumoniae serotypes was evaluated using solid phase microextraction (SPME) and two dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOFMS). Here, seven serotypes (6B, 14, 15, 18C, 19F, 9V, and 23F) were analyzed in an isogenic background. We identified 13 core molecules associated with all seven serotypes, and seven molecules that were differentially produced between serotypes. Serotype 14 was found to have the most distinct volatile profile, and could be discriminated from the other six serotypes in aggregate with an area under the curve (AUC) of 89%. This study suggests that molecules from S. pneumoniae culture headspace show potential for rapid serotype identification.
本研究采用固相微萃取（SPME）和二维气相色谱飞行时间质谱联用（GC×GC-TOFMS）技术评估肺炎链球菌血清型的挥发性分子谱。在此，在同基因背景下分析了七种血清型（6B、14、15、18C、19F、9V 和 23F）。我们鉴定出与所有七种血清型相关的 13 种核心分子，以及七种在血清型之间差异产生的分子。14 血清型被发现具有最独特的挥发性特征，并且可以与其他六种血清型进行区分，总曲线下面积（AUC）为 89%。本研究表明，肺炎链球菌培养顶空的分子具有快速血清型鉴定的潜力。
